Harrison L E, Port J L, Hochwald S, Blumberg D, Burt M
Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
J Surg Res. 1995 Jun;58(6):646-50. doi: 10.1006/jsre.1995.1102.
Administration of perioperative growth hormone may reverse alterations in wound healing and immunologic function in animals receiving chemotherapy. F344 rats were randomized into three groups: Control (n = 12), Chemo (n = 13), and Chemo + GH (n = 12). Human growth hormone (GH) (3 mg/kg sc bid) was begun on Day 0 and continued for 2 weeks. On Day 7, all animals underwent a standardized midline laparotomy, gastrotomy, and placement of a subcutaneous wound sponge. In addition, a single dose of adriamycin (5 mg/kg i.v.) was administered to those animals receiving chemotherapy. On Day 12, right hindlimb footpads were challenged with 50 micrograms of dinitrochlorobenzene. On Day 14, bursting strengths of the laparotomy and gastrotomy were measured. The wound sponge and gastric anastomosis were analyzed for hydroxyproline (OH-Pro) content. Animal spleens were weighed and splenocytes harvested for NK cell activity. Delayed type hypersensitivity (DTH) is reported as percentage of hind-limb foot pad swelling (%FPS). Data are expressed as means +/- SD and comparisons by ANOVA. The laparotomy bursting strength (mm Hg) in the Chemo + GH group (81 +/- 14) was significantly higher than that in the Chemo group (66 +/- 15, P < 0.05). The anastomotic tissue OH-Pro levels (mumole/g dry tissue) in the Chemo + GH group (107.9 +/- 15.2) were significantly higher than those in the Chemo group (62.9 +/- 8.5, P < 0.001). GH increased splenic weights (mg) over those of Chemo (0.50 +/- 0.13 vs 0.37 +/- 0.05, P < 0.05). NK cell activity (% killing) was significantly elevated in the Chemo+GH group compared to that in Chemo.(ABSTRACT TRUNCATED AT 250 WORDS)
围手术期给予生长激素可能会逆转接受化疗动物的伤口愈合和免疫功能改变。将F344大鼠随机分为三组：对照组（n = 12）、化疗组（n = 13）和化疗+生长激素组（n = 12）。于人生长激素（GH）（3mg/kg皮下注射，每日两次）在第0天开始给药，并持续2周。在第7天，所有动物均接受标准的中线剖腹术、胃切开术，并放置皮下伤口海绵。此外，对接受化疗的动物静脉注射单剂量阿霉素（5mg/kg）。在第12天，用50微克二硝基氯苯攻击右后肢足垫。在第14天，测量剖腹术和胃切开术的破裂强度。分析伤口海绵和胃吻合口的羟脯氨酸（OH-Pro）含量。称取动物脾脏重量，并收获脾细胞以检测自然杀伤细胞（NK）活性。迟发型超敏反应（DTH）以后肢足垫肿胀百分比（%FPS）表示。数据以平均值±标准差表示，并通过方差分析进行比较。化疗+生长激素组的剖腹术破裂强度（mmHg）（81±14）显著高于化疗组（66±15，P<0.05）。化疗+生长激素组的吻合组织OH-Pro水平（微摩尔/克干组织）（107.9±15.2）显著高于化疗组（62.9±8.5，P<0.001）。与化疗组相比，生长激素增加了脾脏重量（毫克）（0.50±0.13对0.37±0.05，P<0.05）。与化疗组相比，化疗+生长激素组的NK细胞活性（%杀伤率）显著升高。（摘要截短至250字）
