Takayama C, Nakagawa S, Watanabe M, Mishina M, Inoue Y
Department of Anatomy, Hokkaido University School of Medicine, Sapporo, Japan.
Neurosci Lett. 1995 Mar 24;188(2):89-92. doi: 10.1016/0304-3940(95)11403-j.
The localization of the glutamate receptor channel delta 2 subunit was investigated by immunohistochemistry. The delta 2-immunoreactivity was observed exclusively in the molecular layer of the cerebellar cortex. The electron microscopic analysis showed that the delta 2 subunit was localized in the dendritic spines of the Purkinje cells. The immunopositive spines often formed synaptic contacts with parallel fiber varicosities. As for the Purkinje cells ectopically localized in the cerebellar nuclei and brain stem, the dendritic shafts and cell bodies were strongly labeled. These ectopic Purkinje cells also formed asymmetrical synapses at the delta 2-immunopositive dendritic spines. Considering the specific localization of the delta 2 subunit in the postsynaptic site of the Purkinje cells, the subunit is suggested to be involved in the excitatory synaptic transmission in the cells, as a component of the glutamate receptor channel.
通过免疫组织化学研究了谷氨酸受体通道δ2亚基的定位。仅在小脑皮质分子层观察到δ2免疫反应性。电子显微镜分析表明，δ2亚基定位于浦肯野细胞的树突棘中。免疫阳性的树突棘常与平行纤维膨体形成突触联系。至于异位定位于小脑核和脑干的浦肯野细胞，其树突干和细胞体被强烈标记。这些异位浦肯野细胞也在δ2免疫阳性的树突棘处形成不对称突触。考虑到δ2亚基在浦肯野细胞突触后位点的特定定位，提示该亚基作为谷氨酸受体通道的一个组成部分，参与细胞内的兴奋性突触传递。
