Rohwedder A, Schütz K I, Minamoto N, Brüssow H
Institute of Medical Microbiology and Virology, Ruhr-Universität Bochum, Germany.
Virology. 1995 Jun 20;210(1):231-5. doi: 10.1006/viro.1995.1338.
Partial-length gene 4 cDNA encompassing the VP8* portion of VP4 from chicken rotavirus (RV) Ch-1, turkey RV Ty-1 and Ty-3, and pigeon RV PO-13 was sequenced and compared to RV 993/83 isolated from a calf with diarrhea. Ninety-six percent amino acid sequence identity was seen between VP8* from calf RV 993/83 and pigeon RV PO-13, while only 77 to 83% identity was seen in comparison with turkey and chicken RV VP8* sequences. Phylogenetic tree analysis places all avian RV (including calf isolate 993/83) on a branch at the bottom of the VP8* tree. When tested with three neutralizing monoclonal antibodies raised against pigeon RV PO-13, RV 993/83 and Ty-1 share three, Ch-1 shares two, and Ty-3 shares one neutralizing epitope(s) on VP4 with pigeon RV PO-13.
对包含来自鸡轮状病毒（RV）Ch-1、火鸡RV Ty-1和Ty-3以及鸽RV PO-13的VP4的VP8部分的全长基因4 cDNA进行了测序，并与从腹泻小牛分离的RV 993/83进行了比较。小牛RV 993/83的VP8与鸽RV PO-13之间的氨基酸序列同一性为96%，而与火鸡和鸡RV VP8序列相比，同一性仅为77%至83%。系统发育树分析将所有禽RV（包括小牛分离株993/83）置于VP8树底部的一个分支上。当用针对鸽RV PO-13产生的三种中和单克隆抗体进行测试时，RV 993/83和Ty-1与鸽RV PO-13在VP4上共有三个中和表位，Ch-1共有两个，Ty-3共有一个中和表位。
