Wenger R H, Kopf M, Nitschke L, Lamers M C, Köhler G, Nielsen P J
Max Planck Institut für Immunobiologie, Freiburg, Germany.
Transgenic Res. 1995 May;4(3):173-83. doi: 10.1007/BF01968782.
The murine differentiation marker heat stable antigen (HSA) is a GPI-anchored surface glycoprotein showing strong expression on immature B- and T-lymphocytes and gradually reduced expression during maturation. Although HSA has been suggested to be involved in adhesion and/or signalling, its function has not been clearly demonstrated so far. In order to elucidate the function of HSA, we analysed chimaeric mice that were generated by targeted disruption of both HSA alleles in ES cells. These mice contain normal numbers of peripheral B-cells and normal serum IgM and IgG titres of ES cell-derived allotype, demonstrating that HSA expression on B-cells is not an absolute requirement for their maturation. However, a reduction in immature B-cells in the bone marrow and an altered degree of bone marrow and blood chimaerism suggest that HSA expression influences the maturation of B-cells.
小鼠分化标志物热稳定抗原（HSA）是一种糖基磷脂酰肌醇（GPI）锚定的表面糖蛋白，在未成熟的B淋巴细胞和T淋巴细胞上有强表达，而在成熟过程中表达逐渐降低。尽管有人提出HSA参与黏附及/或信号传导，但到目前为止其功能尚未得到明确证实。为了阐明HSA的功能，我们分析了通过对胚胎干细胞（ES细胞）中两个HSA等位基因进行靶向破坏而产生的嵌合小鼠。这些小鼠外周B细胞数量正常，血清IgM和ES细胞来源的同种异型IgG滴度正常，这表明B细胞上的HSA表达并非其成熟的绝对必要条件。然而，骨髓中未成熟B细胞数量减少以及骨髓和血液嵌合程度改变，提示HSA表达影响B细胞的成熟。
