Chung K T
Appl Environ Microbiol. 1976 Mar;31(3):342-8. doi: 10.1128/aem.31.3.342-348.1976.
Hydrogen inhibits the growth of hydrogen-producing Clostridium cellobioparum, but not of Escherichia coli or Bacteroides ruminicola. The inhibition is reversible. When hydrogen was removed either by palladium black or by gassing out the tube, glucose utilization increased as did optical density and hydrogen production of C. cellobioparum. Removal of the H2 by methanogenic bacteria favors the growth of C. cellobioparum. Grown with Methanobacterium ruminantium in various concentrations of glucose, the Clostridium reaches a higher optical density and produces more H2 and a higher viable cell count. The cell yield is also higher than in pure culture. In mixed culture, C. cellobioparum produces more acetic acid and less lactic acid, ethanol, and butyric acid than in pure culture. The significance of this metabolic shift and hydrogen utilization in methanogenesis is discussed.
氢气可抑制产氢的纤维二糖梭菌的生长，但对大肠杆菌或反刍拟杆菌则无抑制作用。这种抑制是可逆的。当通过钯黑或排出管内气体去除氢气时，纤维二糖梭菌的葡萄糖利用率增加，其光密度和氢气产量也随之增加。产甲烷细菌去除氢气有利于纤维二糖梭菌的生长。在不同浓度葡萄糖条件下与反刍甲烷杆菌共同培养时，梭菌的光密度更高，产生更多氢气，活菌数也更高。细胞产量也高于纯培养。在混合培养中，纤维二糖梭菌比纯培养时产生更多乙酸，而乳酸、乙醇和丁酸的产量则减少。本文讨论了这种代谢转变以及氢气利用在甲烷生成中的意义。
