两种转录因子Gln3p和Nil1p利用相同的GATAAG位点来激活酿酒酵母GAP1的表达。
Two transcription factors, Gln3p and Nil1p, use the same GATAAG sites to activate the expression of GAP1 of Saccharomyces cerevisiae.
作者信息
Stanbrough M, Magasanik B
机构信息
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
出版信息
J Bacteriol. 1996 Apr;178(8):2465-8. doi: 10.1128/jb.178.8.2465-2468.1996.
Abstract
We present an analysis of the DNA region located upstream of GAP1, the structural gene for the general amino acid permease, which contains the sites required for activation of transcription of this gene in response to the nitrogen source of the growth medium. This gene is not expressed in media containing glutamine, and its transcription is activated in response to Gln3p in cells using glutamate as the source of nitrogen and by Nil1p in cells using urea as the source of nitrogen. We show that full response to both activators requires the presence of two GATAAG sites, as well as the presence of auxiliary sites located in the interval between 602 and 453 bp from the translational start site. The fact that both Gln3p and Nil1p utilize GATAAG sites to activate transcription is reflected in the high homology of the zinc finger regions of the two proteins.
摘要
我们对位于通用氨基酸通透酶结构基因GAP1上游的DNA区域进行了分析，该区域包含该基因响应生长培养基氮源而激活转录所需的位点。该基因在含有谷氨酰胺的培养基中不表达，在以谷氨酸为氮源的细胞中其转录由Gln3p激活，在以尿素为氮源的细胞中由Nil1p激活。我们表明，对两种激活剂的完全响应需要存在两个GATAAG位点，以及位于距翻译起始位点602至453 bp区间内的辅助位点。Gln3p和Nil1p都利用GATAAG位点激活转录这一事实反映在这两种蛋白质锌指区域的高度同源性上。
相似文献
1
Two transcription factors, Gln3p and Nil1p, use the same GATAAG sites to activate the expression of GAP1 of Saccharomyces cerevisiae.两种转录因子Gln3p和Nil1p利用相同的GATAAG位点来激活酿酒酵母GAP1的表达。
J Bacteriol. 1996 Apr;178(8):2465-8. doi: 10.1128/jb.178.8.2465-2468.1996.
2
Role of the GATA factors Gln3p and Nil1p of Saccharomyces cerevisiae in the expression of nitrogen-regulated genes.酿酒酵母的GATA因子Gln3p和Nil1p在氮调节基因表达中的作用。
Proc Natl Acad Sci U S A. 1995 Oct 10;92(21):9450-4. doi: 10.1073/pnas.92.21.9450.
3
Role of GATA factor Nil2p in nitrogen regulation of gene expression in Saccharomyces cerevisiae.GATA因子Nil2p在酿酒酵母基因表达氮调控中的作用。
J Bacteriol. 1997 Jun;179(11):3761-6. doi: 10.1128/jb.179.11.3761-3766.1997.
4
The minimal transactivation region of Saccharomyces cerevisiae Gln3p is localized to 13 amino acids.酿酒酵母Gln3p的最小反式激活区域定位于13个氨基酸。
J Bacteriol. 1997 Dec;179(24):7644-52. doi: 10.1128/jb.179.24.7644-7652.1997.
5
Saccharomyces cerevisiae GATA sequences function as TATA elements during nitrogen catabolite repression and when Gln3p is excluded from the nucleus by overproduction of Ure2p.酿酒酵母GATA序列在氮代谢物阻遏期间以及当通过过量表达Ure2p将Gln3p排除在细胞核外时发挥TATA元件的功能。
J Biol Chem. 2000 Jun 9;275(23):17611-8. doi: 10.1074/jbc.M001648200.
6
Interaction of the GATA factor Gln3p with the nitrogen regulator Ure2p in Saccharomyces cerevisiae.酿酒酵母中GATA因子Gln3p与氮调节因子Ure2p的相互作用。
J Bacteriol. 1996 Aug;178(15):4734-6. doi: 10.1128/jb.178.15.4734-4736.1996.
7
Two mutually exclusive regulatory systems inhibit UASGATA, a cluster of 5'-GAT(A/T)A-3' upstream from the UGA4 gene of Saccharomyces cerevisiae.两个相互排斥的调控系统抑制UASGATA，它是酿酒酵母UGA4基因上游的一组5'-GAT(A/T)A-3'序列。
Nucleic Acids Res. 1995 Feb 25;23(4):558-64. doi: 10.1093/nar/23.4.558.
8
A co-activator of nitrogen-regulated transcription in Saccharomyces cerevisiae.酿酒酵母中氮调节转录的一种共激活因子。
Mol Microbiol. 1999 Feb;31(3):753-62. doi: 10.1046/j.1365-2958.1999.01187.x.
9
Gln3p and Nil1p regulation of invertase activity and SUC2 expression in Saccharomyces cerevisiae.酿酒酵母中Gln3p和Nil1p对蔗糖酶活性及SUC2表达的调控
FEMS Yeast Res. 2005 Apr;5(6-7):605-9. doi: 10.1016/j.femsyr.2004.11.011.
10
Urmylation controls Nil1p and Gln3p-dependent expression of nitrogen-catabolite repressed genes in Saccharomyces cerevisiae.泛素相关修饰作用调控酿酒酵母中氮分解代谢阻遏基因的Nil1p和Gln3p依赖性表达。
FEBS Lett. 2007 Feb 6;581(3):541-50. doi: 10.1016/j.febslet.2007.01.019. Epub 2007 Jan 18.
引用本文的文献
1
Engineering artificial cross-species promoters with different transcriptional strengths.构建具有不同转录强度的人工跨物种启动子。
Synth Syst Biotechnol. 2024 Aug 8;10(1):49-57. doi: 10.1016/j.synbio.2024.08.003. eCollection 2025.
2
Paths to adaptation under fluctuating nitrogen starvation: The spectrum of adaptive mutations in Saccharomyces cerevisiae is shaped by retrotransposons and microhomology-mediated recombination.在氮饥饿波动条件下的适应途径：酿酒酵母适应突变的范围是由逆转录转座子和微同源介导的重组形成的。
PLoS Genet. 2023 May 16;19(5):e1010747. doi: 10.1371/journal.pgen.1010747. eCollection 2023 May.
3
The C2H2 Zinc Finger Protein MaNCP1 Contributes to Conidiation through Governing the Nitrate Assimilation Pathway in the Entomopathogenic Fungus .C2H2型锌指蛋白MaNCP1通过调控昆虫病原真菌中的硝酸盐同化途径促进分生孢子形成。
J Fungi (Basel). 2022 Sep 7;8(9):942. doi: 10.3390/jof8090942.
4
Transcription-dependent spreading of the Dal80 yeast GATA factor across the body of highly expressed genes.转录依赖的 Dal80 酵母 GATA 因子在高度表达基因体上的散布。
PLoS Genet. 2019 Feb 28;15(2):e1007999. doi: 10.1371/journal.pgen.1007999. eCollection 2019 Feb.
5
Single-cell copy number variant detection reveals the dynamics and diversity of adaptation.单细胞拷贝数变异检测揭示了适应性的动态和多样性。
PLoS Biol. 2018 Dec 18;16(12):e3000069. doi: 10.1371/journal.pbio.3000069. eCollection 2018 Dec.
6
Systematic identification of factors mediating accelerated mRNA degradation in response to changes in environmental nitrogen.系统鉴定环境氮变化时介导 mRNA 加速降解的因素。
PLoS Genet. 2018 May 21;14(5):e1007406. doi: 10.1371/journal.pgen.1007406. eCollection 2018 May.
7
Dal81 Regulates Expression of Arginine Metabolism Genes in .Dal81调控精氨酸代谢基因在……中的表达 。 （原文最后有个不完整的“in.”，推测应该是某个具体位置，这里只能照原样翻译了）
mSphere. 2018 Mar 7;3(2). doi: 10.1128/mSphere.00028-18. eCollection 2018 Mar-Apr.
8
Achieving global perfect homeostasis through transporter regulation.通过转运体调节实现全球完美的内环境稳定。
PLoS Comput Biol. 2017 Apr 17;13(4):e1005458. doi: 10.1371/journal.pcbi.1005458. eCollection 2017 Apr.
9
Regulation of Nitrogen Metabolism by GATA Zinc Finger Transcription Factors in .GATA锌指转录因子对氮代谢的调控作用于…… （原文结尾不完整，翻译可能不太准确）
mSphere. 2017 Feb 15;2(1). doi: 10.1128/mSphere.00038-17. eCollection 2017 Jan-Feb.
10
Characterization of the Amino Acid Permease Family: Gap2 Is the Only General Amino Acid Permease and Gap4 Is an -Adenosylmethionine (SAM) Transporter Required for SAM-Induced Morphogenesis.氨基酸通透酶家族的特性：Gap2是唯一的通用氨基酸通透酶，Gap4是S-腺苷甲硫氨酸（SAM）诱导形态发生所需的SAM转运体。
mSphere. 2016 Dec 21;1(6). doi: 10.1128/mSphere.00284-16. eCollection 2016 Nov-Dec.
本文引用的文献
1
Transcriptional and posttranslational regulation of the general amino acid permease of Saccharomyces cerevisiae.酿酒酵母通用氨基酸通透酶的转录和翻译后调控
J Bacteriol. 1995 Jan;177(1):94-102. doi: 10.1128/jb.177.1.94-102.1995.
2
Recognition of nitrogen-responsive upstream activation sequences of Saccharomyces cerevisiae by the product of the GLN3 gene.GLN3基因产物对酿酒酵母氮响应上游激活序列的识别。
J Bacteriol. 1995 Jul;177(14):4190-3. doi: 10.1128/jb.177.14.4190-4193.1995.
3
Genetic evidence for Gln3p-independent, nitrogen catabolite repression-sensitive gene expression in Saccharomyces cerevisiae.酿酒酵母中不依赖Gln3p的、对氮代谢物阻遏敏感的基因表达的遗传证据。
J Bacteriol. 1995 Dec;177(23):6910-8. doi: 10.1128/jb.177.23.6910-6918.1995.
4
Role of the GATA factors Gln3p and Nil1p of Saccharomyces cerevisiae in the expression of nitrogen-regulated genes.酿酒酵母的GATA因子Gln3p和Nil1p在氮调节基因表达中的作用。
Proc Natl Acad Sci U S A. 1995 Oct 10;92(21):9450-4. doi: 10.1073/pnas.92.21.9450.
5
Transformation of intact yeast cells treated with alkali cations.经碱金属阳离子处理的完整酵母细胞的转化
J Bacteriol. 1983 Jan;153(1):163-8. doi: 10.1128/jb.153.1.163-168.1983.
6
Adenovirus-2 E1A products repress enhancer-induced stimulation of transcription.腺病毒2型E1A产物可抑制增强子诱导的转录激活。
Nature. 1984;312(5995):608-12. doi: 10.1038/312608a0.
7
Mutational analysis of upstream activation sequence 2 of the CYC1 gene of Saccharomyces cerevisiae: a HAP2-HAP3-responsive site.酿酒酵母CYC1基因上游激活序列2的突变分析：一个HAP2 - HAP3反应位点。
Mol Cell Biol. 1988 Feb;8(2):647-54. doi: 10.1128/mcb.8.2.647-654.1988.
8
Multiple factors bind the upstream activation sites of the yeast enolase genes ENO1 and ENO2: ABFI protein, like repressor activator protein RAP1, binds cis-acting sequences which modulate repression or activation of transcription.多种因素与酵母烯醇化酶基因ENO1和ENO2的上游激活位点结合：ABFI蛋白与阻遏激活蛋白RAP1一样，能结合顺式作用序列，这些序列可调节转录的抑制或激活。
Mol Cell Biol. 1990 Sep;10(9):4872-85. doi: 10.1128/mcb.10.9.4872-4885.1990.
9
Recovery of plasmids from yeast into Escherichia coli: shuttle vectors.从酵母中回收质粒并导入大肠杆菌：穿梭载体。
Methods Enzymol. 1991;194:319-29. doi: 10.1016/0076-6879(91)94024-7.
10
The URE2 gene product of Saccharomyces cerevisiae plays an important role in the cellular response to the nitrogen source and has homology to glutathione s-transferases.酿酒酵母的URE2基因产物在细胞对氮源的反应中起重要作用，并且与谷胱甘肽S-转移酶具有同源性。
Mol Cell Biol. 1991 Feb;11(2):822-32. doi: 10.1128/mcb.11.2.822-832.1991.
Zotero 插件