Hynes R O
Biochim Biophys Acta. 1976 Apr 30;458(1):73-107. doi: 10.1016/0304-419x(76)90015-9.
Many of the altered properties of malignant cells are thought to involve alterations in cell surface functions. In order to understand these alterations it is necessary to know more about the molecular structure of the surface. Methods for analyzing surface proteins are discussed and their application to normal and transformed tissue culture cells are reviewed. A number of surface proteins are observed to be altered by transformation. Most of the alterations are reductions in amounts of particular species, although a few proteins do increase. Evidence concerning the reasons for these alterations and the possible functions of some of the molecules is reviewed. Working hypotheses arising from these data are presented and prospects for understanding the physiological changes in terms of molecular effects are discussed. Particular emphasis is placed on the idea that surface molecules are associated in specific non-covalent complexes which are important for their functions.
许多恶性细胞的改变特性被认为涉及细胞表面功能的改变。为了理解这些改变，有必要更多地了解表面的分子结构。本文讨论了分析表面蛋白的方法，并综述了它们在正常和转化的组织培养细胞中的应用。观察到一些表面蛋白因转化而发生改变。大多数改变是特定种类蛋白数量的减少，不过也有少数蛋白数量增加。本文综述了关于这些改变原因及一些分子可能功能的证据。提出了基于这些数据的工作假说，并讨论了从分子效应角度理解生理变化的前景。特别强调了表面分子以特定非共价复合物形式存在这一观点，这些复合物对其功能很重要。
