Dubinin K V, Zakharova L A, Khegai L A, Zaitsev S V
Division of Chemical Enzymology, Lomonosov Moscow State University, Russia.
Immunopharmacol Immunotoxicol. 1994 Aug;16(3):463-72. doi: 10.3109/08923979409007105.
Met-enkephalin (ME) in the range of concentrations 10(-15) M to 10(-9) M has exhibited an immunomodulating effect on concanavalin A (Con A) induced proliferation of mice lymphocytes from lymph nodes in vitro. The effect of ME was shown to vary with the stage of lymphocyte activation and to depend on the mitogen dose. In the case that ME and Con A were injected simultaneously, at the zero time of proliferation, maximum inhibition was observed on the first and fourth days. In an other set of experiments when ME was added at intervals of 4 hours before measuring the proliferation response value, both inhibition (after 24 hours of proliferation) and stimulation (after 28 and 96 hours) were observed. Opioid ligands of various classes were found to act in the same manner as ME. Naloxone was shown to block the immunomodulating effect of opioids.
浓度在10⁻¹⁵ M至10⁻⁹ M范围内的甲硫氨酸脑啡肽（ME）对体外伴刀豆球蛋白A（Con A）诱导的小鼠淋巴结淋巴细胞增殖具有免疫调节作用。结果表明，ME的作用随淋巴细胞激活阶段的不同而变化，并取决于促有丝分裂原剂量。在ME和Con A同时注射的情况下，即在增殖的零时间，在第一天和第四天观察到最大抑制作用。在另一组实验中，当在测量增殖反应值前每隔4小时添加ME时，观察到了抑制作用（增殖24小时后）和刺激作用（增殖28小时和96小时后）。发现各类阿片样物质配体的作用方式与ME相同。已证明纳洛酮可阻断阿片类药物的免疫调节作用。
