Tomita T, Kashiwagi N, Shimaoka Y, Ikawa T, Tanabe M, Nakagawa S, Kawamura S, Denno K, Owaki H, Ochi T
Department of Environmental Medicine, Osaka University Medical School, Japan.
J Rheumatol. 1994 Sep;21(9):1608-14.
Our previous study showed the presence of abnormal myeloid lineage cells in the epiphyseal bone marrow adjacent to joints affected with severe rheumatoid arthritis (RA). Now, we investigated whether there were any changes of other marrow cell populations related to RA, and whether there were any pathologically characteristic changes in the iliac bone marrow, which is one of the major systemic hematopoietic organs.
2-Color flow cytometry was carried out to analyze the phenotypes of mononuclear cells (MNC) fractions in bone marrow aspirates and venous blood from 56 patients with RA and 7 non-RA controls.
The absolute number of MNC in the iliac bone marrow was increased by 3-fold in the RA patients compared with the non-RA controls. In contrast, no significant increase of MNC was observed in the tibial epiphyseal bone marrow or peripheral blood. The ratio of each MNC fraction in the iliac bone marrow did not differ significantly between the RA patients and the non-RA controls. In lymphocyte subsets, the percentage of HLA-DR+CD8+ cells to all CD8 cells in the iliac bone marrow increased significantly in the RA patients compared with the non-RA controls. Abnormal myeloid cells (MX-GA+MY4+ cells), specific to severe RA, were found to be more concentrated in the iliac bone marrow than in the tibial epiphyseal bone marrow.
Characteristic pathologic changes of the iliac bone marrow suggest an important role of systemic bone marrow in the progression of RA.
我们之前的研究显示，在患有严重类风湿关节炎（RA）的关节附近的骨骺骨髓中存在异常髓系细胞。现在，我们研究了与RA相关的其他骨髓细胞群是否有任何变化，以及作为主要全身造血器官之一的髂骨骨髓是否有任何病理特征性变化。
对56例RA患者和7例非RA对照者的骨髓穿刺液和静脉血中的单核细胞（MNC）组分进行双色流式细胞术分析，以分析其表型。
与非RA对照者相比，RA患者髂骨骨髓中MNC的绝对数量增加了3倍。相比之下，在胫骨骨骺骨髓或外周血中未观察到MNC显著增加。RA患者和非RA对照者髂骨骨髓中各MNC组分的比例无显著差异。在淋巴细胞亚群中，与非RA对照者相比，RA患者髂骨骨髓中HLA-DR+CD8+细胞占所有CD8细胞的百分比显著增加。发现特定于严重RA的异常髓系细胞（MX-GA+MY4+细胞）在髂骨骨髓中比在胫骨骨骺骨髓中更集中。
髂骨骨髓的特征性病理变化提示全身骨髓在RA进展中起重要作用。
