Tomita T, Shimaoka Y, Kashiwagi N, Hashimoto H, Kawamura S, Lee S B, Nakagawa S, Shiho O, Hayashida K, Ochi T
Department of Orthopaedic Surgery, Osaka University Medical School, Japan.
J Rheumatol. 1997 Mar;24(3):465-9.
We previously reported the accumulation of abnormal myeloid cell populations reacting with CD14 (MY4) monoclonal antibody in the iliac and epiphyseal bone marrow of patients with severe rheumatoid arthritis (RA). Therefore, we investigated in vitro production and modulation of CD14+ myeloid cells from iliac bone marrow cells.
Mononuclear cells were prepared from iliac bone marrow aspirates from patients with RA. The presence of unusual myeloid cells was assessed by 2 color flow cytometry of cells cultured under various conditions.
Cultured iliac bone marrow cells of patients with severe RA produced 14.7% of CD14+ CD15+ cells on average. Cultures derived from healthy donors and from patients with a milder form of RA produced fewer CD14+ CD15+ cells (< 10%). The production of CD14+ CD15+ cells was enhanced by granulocyte macrophage colony stimulating factor and interleukin 1beta, but inhibited by T lymphocytes.
Production and modulation of CD14+ myeloid cells were observed in iliac bone marrow of patients with severe RA.
我们之前报道过，在重症类风湿关节炎（RA）患者的髂骨和骨骺骨髓中，存在与CD14（MY4）单克隆抗体发生反应的异常髓样细胞群。因此，我们研究了来自髂骨髓细胞的CD14+髓样细胞的体外产生及调控情况。
从RA患者的髂骨髓穿刺物中制备单核细胞。通过对在各种条件下培养的细胞进行双色流式细胞术，评估异常髓样细胞的存在情况。
重症RA患者培养的髂骨髓细胞平均产生14.7%的CD14+CD15+细胞。来自健康供体和病情较轻的RA患者的培养物产生的CD14+CD15+细胞较少（<10%）。粒细胞巨噬细胞集落刺激因子和白细胞介素1β可增强CD14+CD15+细胞的产生，但T淋巴细胞会抑制其产生。
在重症RA患者的髂骨髓中观察到了CD14+髓样细胞的产生及调控情况。
