Early events in lymph nodes during infection with SIV and FIV.

To elucidate the initial pathogenic events in lymphoid organs, the major reservoir of virus in HIV infection, follow-ups of viral load, pathological changes and target cells were performed in the rhesus macaque SIVmac251 model and in the cat FIV model. Lymph nodes (LN) obtained from animals sacrificed at early time points following experimental inoculation were analysed by in situ hybridization for virus load and by combined immunohistochemistry and in situ hybridization for virus cellular tropism. In the SIV model, the LN presented a high viral load at 7 days post inoculation (p.i.); at this stage, macrophages and T4 lymphocytes were identified as the target cells of the virus. A shift in the pattern of viral infection was observed at 2 weeks p.i., with a concentration of viral RNA in follicular dendritic cells (FDC) in the germinal centres of the developing lymphoid follicles. This FDC-associated virus persisted at high levels for 2 months p.i. in the FIV model, the number of infected cells detected in LN was very low compared with that found in the SIV model, and a similar role played by FDC was found.