Bach J M, Hurtrel M, Chakrabarti L, Ganiere J P, Montagnier L, Hurtrel B
Unité d'Oncologie Virale, Institut Pasteur, Paris, France.
AIDS Res Hum Retroviruses. 1994 Dec;10(12):1731-8. doi: 10.1089/aid.1994.10.1731.
Analysis of the early stages of infection within the lymphoid organs is crucial for the understanding of the physiopathology of HIV infection. Such analysis can only be performed using animal models. Cats were infected with two strains of FIV and killed at regular intervals for a classic pathologic study along with a quantification of the viral load by in situ hybridization in the spleen and the lymph nodes. The pathological study showed a persistent follicular reaction, which peaked 15 days postinoculation (p.i.). The in situ hybridization study showed two types of labeling. The first was spot labeling corresponding to cells actively replicating the virus. The second consisted of a more diffuse labeling linked to the follicular dendritic cells (FDCs) demonstrating by colocalization of virus detected by in situ hybridization associated with the FDCs, specifically labeled by immunohistochemistry. The number of productive cells is few and identical for the two viruses tested. Despite a slight peak at 15 days p.i., the number of infected cells persists while slightly decreasing over time. The FDC virus load appears jointly with the appearance of antibody and remains permanent until the end of the study at 3 years p.i. These results show that in the FIV model, there is a chronic permanent infection in the lymphoid organs. Furthermore, as compared with the SIV-macaque model, there is a correlation between the low number of infected cells detected in these organs in the early phase and the extended length of the asymptomatic period, which contrasts with the high level of the FDC virus load lasting during the same period.
分析淋巴器官内感染的早期阶段对于理解HIV感染的生理病理学至关重要。这种分析只能使用动物模型来进行。猫感染了两种猫免疫缺陷病毒（FIV）毒株，并每隔一定时间处死，进行经典病理学研究以及通过原位杂交对脾脏和淋巴结中的病毒载量进行定量分析。病理学研究显示出持续的滤泡反应，在接种后15天达到峰值。原位杂交研究显示出两种标记类型。第一种是与积极复制病毒的细胞相对应的点状标记。第二种是与滤泡树突状细胞（FDC）相关的更弥漫性标记，通过原位杂交检测到的病毒与FDC的共定位证明，FDC通过免疫组织化学特异性标记。对于所测试的两种病毒，产生病毒的细胞数量很少且相同。尽管在接种后15天出现了轻微峰值，但受感染细胞的数量持续存在，同时随时间略有下降。FDC病毒载量与抗体的出现同时出现，并一直持续到接种后3年研究结束。这些结果表明，在FIV模型中，淋巴器官存在慢性持续性感染。此外，与猴免疫缺陷病毒（SIV）-猕猴模型相比，在这些器官早期检测到的受感染细胞数量较少与无症状期的延长之间存在相关性，这与同期持续的高水平FDC病毒载量形成对比。
