Marinovich M, Guizzetti M, Galli C L
Laboratory of Toxicology, University of Milan, Italy.
Toxicology. 1994 Nov-Dec;94(1-3):173-85. doi: 10.1016/0300-483x(94)90036-1.
Dimethoate, azinphos-methyl, diazinon and pirimiphos-methyl, widely used organophosphorous insecticides, and benomyl, a benzimidazole fungicide, induce different cytotoxic effects on the human leukemia cell line HL-60. Among the insecticides tested, only azinphos and diazinon induced a dose-related inhibition of protein synthesis in HL-60 cells at 24 h, at 60 and 40 micrograms/ml medium, respectively. Dimethoate and pirimiphos were not active up to 100 micrograms/ml. Benomyl strongly inhibited protein synthesis at 50 micrograms/ml and the polymerisation of actin to give cytoskeletal microfilaments (F-actin) at 30 micrograms/ml. Mixtures of benomyl-pirimiphos and dimethoate azinphos-diazinon were also investigated. Pirimiphos, when present in equal concentration, antagonized the inhibitory effect of benomyl on protein synthesis at 4 h, but not at 24 h. The effect of the other insecticide mixture on the same parameter was greater than that of the two active components, diazinon and azinphos given singly.
乐果、甲基谷硫磷、二嗪农和甲基嘧啶磷是广泛使用的有机磷杀虫剂，苯菌灵是一种苯并咪唑类杀菌剂，它们对人白血病细胞系HL-60具有不同的细胞毒性作用。在所测试的杀虫剂中，只有谷硫磷和二嗪农在24小时时分别在培养基浓度为60和40微克/毫升时对HL-60细胞的蛋白质合成产生剂量相关的抑制作用。乐果和甲基嘧啶磷在高达100微克/毫升时没有活性。苯菌灵在50微克/毫升时强烈抑制蛋白质合成，在30微克/毫升时抑制肌动蛋白聚合成细胞骨架微丝（F-肌动蛋白）。还研究了苯菌灵-甲基嘧啶磷和乐果-谷硫磷-二嗪农的混合物。当甲基嘧啶磷以相同浓度存在时，在4小时而非24小时时拮抗苯菌灵对蛋白质合成的抑制作用。另一种杀虫剂混合物对同一参数的影响大于单独使用的两种活性成分二嗪农和谷硫磷的影响。
