Bianchi L, Zannoli A, Pizzala R, Stivala L A, Chiesara E
Istituto di Patologia Generale, Università di Pavia, Italy.
Mutat Res. 1994 Jun;321(4):203-11. doi: 10.1016/0165-1218(94)90071-x.
Four organophosphorus pesticides (azinphos-methyl, diazinone, dimethoate, and pirimiphos-methyl), and one carbamate (benomyl) were tested for cytotoxicity, reverse mutation and gene conversion in Saccharomyces cerevisiae D7, with and without the S9 metabolic system. Furthermore, two mixtures of the above compounds, namely benomyl + pirimiphos-methyl (6/1 ratio) and dimethoate + diazinone + azinphos-methyl (10/4/6 ratio) were tested in the same experimental model. Azinphos-methyl, benomyl, and pirimiphos-methyl alone did not induce any genotoxic effect, whereas azinphos-methyl and diazinone were active in inducing reversion and gene conversion. The benomyl + pirimiphos-methyl mixture did not show any genotoxic activity. The dimethoate + diazinone + azimphos-methyl mixture was genotoxic, although an antagonistic effect between the components was observed. The addition of S9 post-mitochondrial liver fraction decreased the activity of both single and mixed genotoxic agents.
在有和没有S9代谢系统的情况下，对酿酒酵母D7测试了四种有机磷农药（甲基谷硫磷、二嗪磷、乐果和甲基嘧啶磷）以及一种氨基甲酸酯（苯菌灵）的细胞毒性、回复突变和基因转换。此外，在同一实验模型中测试了上述化合物的两种混合物，即苯菌灵 + 甲基嘧啶磷（6/1比例）和乐果 + 二嗪磷 + 甲基谷硫磷（10/4/6比例）。单独的甲基谷硫磷、苯菌灵和甲基嘧啶磷未诱导任何遗传毒性效应，而甲基谷硫磷和二嗪磷在诱导回复突变和基因转换方面具有活性。苯菌灵 + 甲基嘧啶磷混合物未显示任何遗传毒性活性。乐果 + 二嗪磷 + 甲基谷硫磷混合物具有遗传毒性，尽管观察到各成分之间存在拮抗作用。添加S9线粒体后肝组分降低了单一和混合遗传毒性剂的活性。
