Arato A, Savilahti E, Paszti I
1st Department of Paediatrics, Semmelweis Medical University, Budapest, Hungary.
Z Gastroenterol. 1994 Sep;32(9):483-7.
The presence of cells positive for interleukin-2 receptors (CD25) and the proportion of Ki-67 positive dividing cells in the crypts were studied with monoclonal antibodies using sensitive immunohistochemical techniques in the rectal biopsy specimens taken from 13 children with inflammatory bowel disease (4 Crohn's disease and 9 ulcerative colitis) and 10 controls. In all specimens, but one, from patients CD25+ cells were found in the surface and crypt epithelium, while in none of controls were seen such cells. The mean percentage of dividing crypt cells was significantly higher in ulcerative colitis (36.0%) and in Crohn's disease (34.6%) than in controls (18.4) (p < 0.001 in both comparisons). In ulcerative colitis, a correlation between CD25/CD3 ratios in the surface and crypt epithelium and the percentage of dividing crypt cells was found. We conclude that activated lymphocytes have a role in the crypt hyperplasia of ulcerative colitis.
采用敏感的免疫组织化学技术，使用单克隆抗体对13例炎症性肠病患儿（4例克罗恩病和9例溃疡性结肠炎）及10例对照者的直肠活检标本进行研究，观察白细胞介素-2受体（CD25）阳性细胞的存在情况以及隐窝中Ki-67阳性分裂细胞的比例。在所有患者（仅1例除外）的标本中，表面和隐窝上皮均发现了CD25+细胞，而在所有对照者标本中均未见到此类细胞。溃疡性结肠炎（36.0%）和克罗恩病（34.6%）中隐窝分裂细胞的平均百分比显著高于对照组（18.4%）（两项比较的p值均<0.001）。在溃疡性结肠炎中，表面和隐窝上皮的CD25/CD3比值与隐窝分裂细胞百分比之间存在相关性。我们得出结论，活化淋巴细胞在溃疡性结肠炎的隐窝增生中起作用。
