Tofanelli S, Stanyon R, Agostini M, Franceschi M G, Paoli G
Istituto di Antropologia, Università di Firenze, Italy.
Hum Biol. 1993 Aug;65(4):635-46.
DA/DAPI and C chromosomal heteromorphic sites (1q, 9q, 15p, 16q, Yq) in a sample of 136 unrelated individuals from the Garfagnana valley (Tuscany, Italy) have been analyzed quantitatively and qualitatively. The variations in length, between-homolog heteromorphisms, and intensity of fluorescence of the heterochromatic bands have been compared in two subsamples of the population (upper and middle valley) individualized according to geodemographic criteria. DA/DAPI heterochromatin differed significantly from C heterochromatin, showing a lower average amount and a higher variability at each site. This suggests a differential staining of DNA of the two banding systems. Furthermore, DA/DAPI heterochromatin was less uniformly distributed in the population than C heterochromatin and the regions 16q and Yq discriminated better between subsamples. The variations of DA/DAPI fluorescence at the 15p site demonstrated an excess of homomorphic individuals in the upper valley, which could be related to the mating structure of the population living in this area.
对来自意大利托斯卡纳加尔法尼亚纳山谷的136名无亲缘关系个体的样本，进行了DA/DAPI和C染色体异态位点（1q、9q、15p、16q、Yq）的定量和定性分析。根据地理人口统计学标准划分出该人群的两个子样本（山谷上部和中部），比较了异染色质带的长度变化、同源染色体间的异态性以及荧光强度。DA/DAPI异染色质与C异染色质有显著差异，每个位点的平均含量较低且变异性较高。这表明两种带型系统的DNA染色存在差异。此外，DA/DAPI异染色质在人群中的分布不如C异染色质均匀，16q和Yq区域在子样本间的区分度更好。15p位点DA/DAPI荧光的变化表明山谷上部同态个体过多，这可能与该地区人群的交配结构有关。
