Sutovský P, Fléchon J E, Pavlok A
Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Libĕchov.
Reprod Nutr Dev. 1994;34(5):415-25. doi: 10.1051/rnd:19940503.
The relationship between cytoskeleton and morphology of cumulus granulosa cells in expanding bovine oocyte-cumulus complexes (OCCs) cultured in vitro has been investigated by the means of indirect immunofluorescence and transmission electron microscopy. The round-shaped cells in unstimulated control OCCs displayed a homogeneous distribution of cytoskeletal networks and cytoplasmic organelles. Luteinizing hormone (LH) stimulation caused the redistribution of microfilaments (MFs), accelerated the development of Golgi apparatus, and led to the generation of lipid droplets in cumulus cells. These changes culminated in the elongation and polarization of cumulus cells and in the extension of the cytoplasmic networks of microtubules (MTs) and intermediate filaments (IFs) into the newly formed cytoplasmic projections. The culture of OCCs in the presence of microfilament disruptor cytochalasin B prevented cumulus expansion, formation of cellular projections and cell elongation and suppressed the development of the Golgi apparatus. On the contrary, cytochalasin had no effect on the abundance and distribution of lipid droplets and on the integrity of IFs and MTs. The present data support the hypothesis that the response of cumulus granulosa cells to LH is partially mediated by F-actin.
通过间接免疫荧光和透射电子显微镜技术，研究了体外培养的扩张期牛卵丘-卵母细胞复合体（OCCs）中细胞骨架与卵丘颗粒细胞形态之间的关系。未受刺激的对照OCCs中的圆形细胞显示细胞骨架网络和细胞质细胞器分布均匀。促黄体生成素（LH）刺激导致微丝（MFs）重新分布，加速高尔基体的发育，并导致卵丘细胞中脂滴的产生。这些变化最终导致卵丘细胞伸长和极化，以及微管（MTs）和中间丝（IFs）的细胞质网络延伸到新形成的细胞质突起中。在存在微丝破坏剂细胞松弛素B的情况下培养OCCs可阻止卵丘扩张、细胞突起形成和细胞伸长，并抑制高尔基体的发育。相反，细胞松弛素对脂滴的丰度和分布以及IFs和MTs的完整性没有影响。目前的数据支持这样的假设，即卵丘颗粒细胞对LH的反应部分由F-肌动蛋白介导。
