López Ruiz B, Sánchez-Cabezudo M, Mottola H A
Sección Departamental de Química Analítica, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Analyst. 1996 Nov;121(11):1695-8. doi: 10.1039/an9962101695.
The neuromuscular blocking agent, succinylcholine, and the neurotransmitter, acetylcholine, have been determined in pharmaceutical preparations utilizing a sensing unit comprised of a rotating bioreactor and an amperometric detector. The bioreactor consisted of a rotating disk made of Teflon (1 cm id) with cholinesterase (EC 3.1.1.8) and choline oxidase (EC 1.1.3.17) co-immobilized on the top surface of the disk. Amperometric detection of H2O2 and initial rate measurements were employed for quantification.
利用由旋转生物反应器和安培检测器组成的传感单元，已测定了药物制剂中的神经肌肉阻滞剂琥珀酰胆碱和神经递质乙酰胆碱。生物反应器由聚四氟乙烯制成的旋转圆盘（内径1厘米）组成，胆碱酯酶（EC 3.1.1.8）和胆碱氧化酶（EC 1.1.3.17）共固定在圆盘的顶表面。采用安培法检测过氧化氢并进行初始速率测量以进行定量。
