Beitins I Z, O'Loughlin K, Ostrea T, McArthur J W
J Clin Endocrinol Metab. 1976 Jul;43(1):46-55. doi: 10.1210/jcem-43-1-46.
The usefulness of timed 3-hour urine collections as a substitute for serum gonadotropin (LH and FSH) determinations during the menstrual cycle and during LHRH testing was examined. The timing of the 3-hour urine collection is not important in mature individuals, since no significant temporal trend was found when aliquots were collected every 3 hours throughout two 24-hour periods in one mature woman. Good correlation was found between serum LH and FSH concentrations and the quantity of LH and FSH in timed 3-hour urine specimens throughout normal, ovulatory menstrual cycles in two women. Studies before and during 51 LHRH stimulation tests in normal men, children, and women during different phases of the menstrual cycle and in patients with a variety of hypothalamic-pituitary-gonadal axis disorders were performed. There was good correlation between the quantity of the gonadotropins in time 3-hour urine collections and the mean serum LH and FSH concentrations before and during the LHRH test. The "response area" for serum LH and FSH also correlated well with the amounts of LH and FSH in the urine collected during this period. Therefore, the timed 3-hour urine collection for gonadotropin estimation provides a simple, accurate method for the integration of fluctuating serum concentrations of LH and FSH during such instances of physiologic variability as the menstrual cycle and LHRH stimulation tests.
研究了在月经周期和促黄体生成素释放激素（LHRH）测试期间，定时采集3小时尿液作为血清促性腺激素（促黄体生成素LH和促卵泡生成素FSH）测定替代方法的实用性。在成熟个体中，3小时尿液采集的时间并不重要，因为在一名成熟女性的两个24小时期间内，每隔3小时采集一次等分试样时，未发现明显的时间趋势。在两名女性的正常排卵月经周期中，血清LH和FSH浓度与定时3小时尿液标本中LH和FSH的量之间发现了良好的相关性。对正常男性、儿童和女性在月经周期不同阶段以及患有各种下丘脑-垂体-性腺轴疾病的患者进行了51次LHRH刺激试验之前和期间的研究。在LHRH测试之前和期间，定时3小时尿液采集中促性腺激素的量与平均血清LH和FSH浓度之间存在良好的相关性。血清LH和FSH的“反应区”也与在此期间采集的尿液中LH和FSH的量密切相关。因此，定时采集3小时尿液用于促性腺激素评估，为整合月经周期和LHRH刺激试验等生理变化情况下LH和FSH波动的血清浓度提供了一种简单、准确的方法。
