Mitra A, Guha A K, Chatterjee B P
Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Calcutta.
Zentralbl Bakteriol. 1994 Jun;281(1):55-60. doi: 10.1016/s0934-8840(11)80637-7.
Ten different serogroups of Shigella dysenteriae were typed with the aid of lectins of known sugar specificity resulting from their interactions with the carbohydrates on lipopolysaccharides in the outer membrane of bacteria as evidenced by the agglutination-inhibition assay with simple carbohydrates. Lipopolysaccharides of two serogroups of Shigella were precipitated with different lectins and the results were corroborated by those derived from the agglutination assay suggesting that Shigella dysenteriae can be characterized on the species level with the aid of lectins.
借助已知糖特异性的凝集素,通过与细菌外膜脂多糖上的碳水化合物相互作用,对十种不同血清群的痢疾志贺氏菌进行分型,这一点通过用单糖进行的凝集抑制试验得到了证实。两种血清群的痢疾志贺氏菌脂多糖与不同的凝集素发生沉淀反应,凝集试验结果也证实了这一点,这表明借助凝集素可在种水平上对痢疾志贺氏菌进行特征描述。