Wright L W, Lichter J B, Reinitz J, Shifman M A, Kidd K K, Miller P L
Center for Medical Informatics, Yale University School of Medicine, New Haven, CT 06520-8009.
Comput Appl Biosci. 1994 Jul;10(4):435-42. doi: 10.1093/bioinformatics/10.4.435.
Building a map of restriction sites from double-digest gel data can be a complex and frustrating task, especially when many DNA fragments are detected or when the gel results are ambiguous. 'Double Digester' is an interactive, graphical computer program which helps researchers understand and resolve such data. It explicitly represents the experimental data, the associated uncertainties, the researcher's hypotheses and possible map interpretations. Alternative solutions are frequently possible, and the differences between them may help determine which additional experiments might resolve ambiguities. Initial use has confirmed the benefits of this approach, and has suggested ways in which it can be refined and extended. Double Digester meets the need for a practical tool to help build restriction maps, and also illustrates how a computer-based tool can confront experimental uncertainty in an integrated fashion.
根据双酶切凝胶数据构建限制性酶切位点图谱可能是一项复杂且令人沮丧的任务，尤其是在检测到许多DNA片段或凝胶结果不明确时。“双酶切分析器”是一个交互式图形计算机程序，可帮助研究人员理解和解析此类数据。它明确表示实验数据、相关的不确定性、研究人员的假设以及可能的图谱解释。通常存在多种替代解决方案，它们之间的差异可能有助于确定哪些额外的实验可以解决模糊性问题。初步使用已经证实了这种方法的益处，并提出了可以对其进行改进和扩展的方法。双酶切分析器满足了构建限制性图谱实用工具的需求，同时也说明了基于计算机的工具如何以综合方式应对实验不确定性。
