Lei W D, Zhang R G, Yan S Z
Cancer Hospital, Chinese Academy of Medical Sciences, Beijing.
Zhonghua Zhong Liu Za Zhi. 1994 Jul;16(4):277-9.
Using labelled streptavidin-biotin (LSAB) method, we examined the expression of nucleoside diphosphate kinase(NDPK), the product of metastasis suppressor gene nm23, in human lung cancer. Of 88 patients tested, 48 (54.5%) showed positive staining. The positive staining rate was higher in adenocarcinoma (28/42, 66.7%) than in squamous cell carcinoma (20/46, 43.5%; P < 0.05). Higher incidence of positive staining was also found in squamous cell carcinoma without hilar or mediastinal lymph node metastasis (16/27, 59.3%) than in that with hilar or mediastinal lymph node involvement (4/19, 21.1%; P < 0.05). NDPK/nm23 was equally expressed in adenocarcinoma irrespective of lymph node status. In both cell types of carcinoma, expression of NDPK/nm23 was not correlated with tumor cell differentiation, nor was it correlated with the P-TNM staging. Our results suggest that NDPK/nm23 may play different roles in the pathogenesis and metastasis of human pulmonary squamous cell carcinoma and adenocarcinoma. Its expression levels are inversely correlated with lymph node metastasis in squamous cell carcinoma.
我们采用标记链霉亲和素-生物素（LSAB）法，检测了转移抑制基因nm23的产物核苷二磷酸激酶（NDPK）在人肺癌中的表达。在检测的88例患者中，48例（54.5%）呈阳性染色。腺癌的阳性染色率（28/42，66.7%）高于鳞状细胞癌（20/46，43.5%；P<0.05）。无肺门或纵隔淋巴结转移的鳞状细胞癌阳性染色发生率（16/27，59.3%）也高于有肺门或纵隔淋巴结受累的鳞状细胞癌（4/19，21.1%；P<0.05）。腺癌中NDPK/nm23的表达与淋巴结状态无关。在两种类型的癌中，NDPK/nm23的表达均与肿瘤细胞分化无关，也与P-TNM分期无关。我们的结果表明，NDPK/nm23可能在人肺鳞状细胞癌和腺癌的发病机制及转移中发挥不同作用。其表达水平与鳞状细胞癌的淋巴结转移呈负相关。
