康氏立克次体进入非洲绿猴肾细胞的过程。
Rickettsia conorii entry into Vero cells.
作者信息
Teysseire N, Boudier J A, Raoult D
机构信息
Unité des Rickettsies, Centre National de la Recherche Scientifique EP J 0054, Faculté de Médecine, la Timone, France.
出版信息
Infect Immun. 1995 Jan;63(1):366-74. doi: 10.1128/iai.63.1.366-374.1995.
Abstract
The entry of rickettsiae into eukaryotic cells is mediated by an induced phagocytosis, but rickettsiae have never been observed in a closed phagocytic vacuole. In this study, Rickettsia conorii entry into Vero cells was observed by transmission electron microscopy during a period of 3 to 20 min after bacterium-cell contact. The entry occurred within 3 min after bacterium-cell contact, and R. conorii was observed in the process of engulfment, within a phagocytic vacuole, or free in the cytosol. Escape from the phagosome is a very rapid step since phagosome lysis was only occasionally observed. By 12 min, 90% of bacteria were internalized and half were free in the cytosol. This report confirms that rickettsiae penetrate nonphagocytic cells by induced phagocytosis and is the first demonstration of rickettsiae within a complete phagocytic vacuole.
摘要
立克次氏体进入真核细胞是由诱导吞噬作用介导的，但从未在封闭的吞噬泡中观察到立克次氏体。在本研究中，通过透射电子显微镜观察了在细菌与细胞接触后3至20分钟内，康氏立克次氏体进入Vero细胞的过程。进入发生在细菌与细胞接触后的3分钟内，观察到康氏立克次氏体处于吞噬过程中、吞噬泡内或胞质溶胶中自由存在。从吞噬体逃逸是一个非常迅速的步骤，因为仅偶尔观察到吞噬体裂解。到12分钟时，90%的细菌被内化，一半在胞质溶胶中自由存在。本报告证实立克次氏体通过诱导吞噬作用穿透非吞噬细胞，并且首次证明了立克次氏体存在于完整的吞噬泡中。
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本文引用的文献
1
Centrifugation and Rickettsiae and viruses onto cells and its effect on infection.将立克次氏体和病毒离心到细胞上及其对感染的影响。
Proc Soc Exp Biol Med. 1960 Apr;103:691-5. doi: 10.3181/00379727-103-25637.
2
Fate of Listeria monocytogenes in murine macrophages: evidence for simultaneous killing and survival of intracellular bacteria.单核细胞增生李斯特菌在小鼠巨噬细胞中的命运：细胞内细菌同时被杀伤和存活的证据
Infect Immun. 1994 Feb;62(2):543-53. doi: 10.1128/iai.62.2.543-553.1994.
3
Phospholipase A and the interaction of Rickettsia prowazekii and mouse fibroblasts (L-929 cells).磷脂酶A以及普氏立克次体与小鼠成纤维细胞（L-929细胞）的相互作用。
Infect Immun. 1982 Oct;38(1):109-13. doi: 10.1128/iai.38.1.109-113.1982.
4
In vitro studies of Rickettsia-host cell interactions: ultrastructural study of Rickettsia prowazekii-infected chicken embryo fibroblasts.立克次氏体与宿主细胞相互作用的体外研究：普氏立克次氏体感染鸡胚成纤维细胞的超微结构研究
Infect Immun. 1980 Aug;29(2):778-90. doi: 10.1128/iai.29.2.778-790.1980.
5
Phospholipase A activity in the hemolysis of sheep and human erythrocytes by Rickettsia prowazeki.普氏立克次体对绵羊和人红细胞溶血作用中的磷脂酶A活性。
Infect Immun. 1980 Aug;29(2):316-21. doi: 10.1128/iai.29.2.316-321.1980.
6
Rickettsia rickettsii-induced cellular injury of human vascular endothelium in vitro.立氏立克次体在体外诱导人血管内皮细胞损伤。
Infect Immun. 1984 Jun;44(3):545-53. doi: 10.1128/iai.44.3.545-553.1984.
7
Rickettsial interactions with human endothelial cells in vitro: adherence and entry.立克次体与人类内皮细胞的体外相互作用：黏附与侵入。
Infect Immun. 1984 May;44(2):205-10. doi: 10.1128/iai.44.2.205-210.1984.
8
Role of phospholipase-associated penetration mechanism in cell injury by Rickettsia rickettsii.磷脂酶相关穿透机制在立氏立克次体所致细胞损伤中的作用
Infect Immun. 1983 May;40(2):840-2. doi: 10.1128/iai.40.2.840-842.1983.
9
Injury restricted to cells infected with spotted fever group rickettsiae in parabiotic chambers.损伤仅限于联体动物实验箱中感染斑点热群立克次体的细胞。
Acta Trop. 1984 Dec;41(4):307-12.
10
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Infect Immun. 1986 Feb;51(2):461-9. doi: 10.1128/iai.51.2.461-469.1986.
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