Morita Y, Araki S, Sakai T, Araki T, Masuyama Y
Department of Public Health, Faculty of Medicine, University of Tokyo, Japan.
Ind Health. 1994;32(2):85-96. doi: 10.2486/indhealth.32.85.
We developed a simple and reliable method for determining delta-aminolevulinic acid (ALA) in plasma (ALA-P) by fluorometric high-performance liquid chromatography. The method was applicable to 100 microliters of plasma and the mean +/- standard deviation for analytical recovery was 100.4 +/- 2.6% when 50 micrograms/l of ALA was added to 14 plasma samples. The detection limit for ALA-P was 2.0 micrograms/l (signal-to-noise ratio was 5) and the standard curve was linear in a wide range up to 400 micrograms/l. The ALA-P level for 26 unexposed male adults was 8.6 +/- 1.3 micrograms/l. In 72 male lead workers, logarithm of ALA-P concentration was significantly correlated with blood lead concentrations (Pb-B) of 2.5-115.4 micrograms/dl (r = 0.924, p < 0.001). Even at the Pb-B level less than 15 micrograms/dl, significant linear relationships were found between Pb-B and log ALA-P (r = 0.632, p < 0.01), between Pb-B and delta-aminolevulinic acid dehydratase activity (ALA-D) (r = -0.573, p < 0.05), and between ALA-D and ALA-P (r = -0.765, p < 0.001). It is suggested that ALA-P determined by the present method is a useful indicator of biological effects of lead, especially in the wide range of Pb-B level.
我们开发了一种通过荧光高效液相色谱法测定血浆中δ-氨基乙酰丙酸（ALA）（血浆ALA，即ALA-P）的简单可靠方法。该方法适用于100微升血浆，当向14份血浆样本中添加50微克/升的ALA时，分析回收率的平均值±标准差为100.4±2.6%。血浆ALA-P的检测限为2.0微克/升（信噪比为5），标准曲线在高达400微克/升的宽范围内呈线性。26名未接触铅的成年男性的血浆ALA-P水平为8.6±1.3微克/升。在72名男性铅作业工人中，血浆ALA-P浓度的对数与血铅浓度（Pb-B）在2.5 - 115.4微克/分升之间显著相关（r = 0.924，p < 0.001）。即使在血铅水平低于15微克/分升时，血铅与血浆ALA-P对数之间（r = 0.632，p < 0.01）、血铅与δ-氨基乙酰丙酸脱水酶活性（ALA-D）之间（r = -0.573，p < 0.05）以及ALA-D与血浆ALA-P之间（r = -0.765，p < 0.001）也发现了显著的线性关系。提示用本方法测定的血浆ALA-P是铅生物学效应的有用指标，尤其在血铅水平的广泛范围内。
