Yamamoto N, Sugiura W, Kosaka H, Nakamura S
Department of School Nurse, Osaka Kyoiku University, Japan.
Nihon Eiseigaku Zasshi. 1994 Oct;49(4):791-6. doi: 10.1265/jjh.49.791.
We found that human urine suppressed SOS-responses induced by furylfuramide (AF-2), as detected by the umu test using Salmonella typhimurium TA1535/pSK1002. In the present report, we studied the time stability of the SOS-inhibition activity in urine. The diurnal and daily changes of SOS-inhibition in the urine were also observed. Results obtained were as follows; 1) SOS-inhibition activity of the urine remained stable more than one month after the urine was frozen. 2) Individual variation was observed in the SOS-inhibition activity of the urine. 3) Total SOS-inhibition activity of per a day showed relatively small variation during experimental days. 4) The SOS-inhibition activity of urine was higher early in the morning than in the daytime. The activity fell gradually with time in the daytime and showed the lowest value in the evening. Then, it rose again at night. Therefore, it is necessary to collect urine at specific times to avoid the differences caused by diurnal changes in SOS-inhibition activity.
我们发现，使用鼠伤寒沙门氏菌TA1535/pSK1002进行umu试验检测时，人尿可抑制由呋喃糠酰胺（AF-2）诱导的SOS反应。在本报告中，我们研究了尿中SOS抑制活性的时间稳定性。还观察了尿中SOS抑制的昼夜和每日变化。结果如下：1）尿液冷冻后，其SOS抑制活性在一个多月内保持稳定。2）观察到尿中SOS抑制活性存在个体差异。3）在实验期间，每天的总SOS抑制活性变化相对较小。4）晨尿中的SOS抑制活性高于白天。白天该活性随时间逐渐下降，晚上达到最低值。然后，夜间又再次上升。因此，有必要在特定时间收集尿液，以避免因SOS抑制活性的昼夜变化而导致的差异。
