Wolff K D, Stiller D
Department of Maxillofacial Plastic Surgery, Free University of Berlin, Germany.
Plast Reconstr Surg. 1993 Mar;91(3):485-91. doi: 10.1097/00006534-199303000-00015.
The ischemia tolerance of free-muscle flaps was investigated in the rat by orthotopic and syngeneic transplantation of muscle flaps measuring 4 x 2.5 cm. The effects of ischemia with a maximal duration of 7 hours on the raised flaps and of anoxia caused by occlusion of the flap vessels were studied for the first time using 31P-spectroscopy. Energy metabolism was found to recover fast after 3 hours of ischemia, whereas a duration of 4 hours led to severe, only slowly reversible changes. Longer periods of ischemia resulted in a complete loss of energy reserves (PCr) and weaker ATP signal. The complete absence of ATP and a pH value under 6 indicated irreversible muscular damage.
通过对4×2.5厘米大小的肌肉瓣进行原位和同基因移植，在大鼠身上研究了游离肌肉瓣的缺血耐受性。首次使用31P光谱研究了最长持续7小时的缺血对提起的肌肉瓣的影响以及由肌肉瓣血管闭塞引起的缺氧情况。发现缺血3小时后能量代谢迅速恢复，而缺血4小时会导致严重的、仅缓慢可逆的变化。更长时间的缺血会导致能量储备（磷酸肌酸）完全丧失和ATP信号减弱。ATP完全缺失且pH值低于6表明肌肉发生了不可逆损伤。
