Ingolfsdottir K, Jurcic K, Fischer B, Wagner H
Department of Pharmacy, University of Iceland, Reykjavik.
Planta Med. 1994 Dec;60(6):527-31. doi: 10.1055/s-2006-959564.
A new alkali-soluble polysaccharide has been isolated from Iceland moss, Cetraria islandica (L.) Ach., by ethanol fractionation, ion-exchange chromatography, and gel filtration. The mean M(r) was estimated to be 18,000. Sugar and methylation analysis, partial hydrolysis, and 13C-NMR spectroscopy showed the polysaccharide to be a branched galactomannan with a backbone composed of two structural elements; (1-->6)-linked alpha-D-mannopyranosyl and alpha-D-(1-->6)-galactopyranosyl units. The polysaccharide showed pronounced immunostimulating activity in an in vitro phagocytosis assay and in the in in vivo carbon clearance assay.
通过乙醇分级分离、离子交换色谱法和凝胶过滤法，从冰岛衣（Cetraria islandica (L.) Ach.）中分离出一种新的碱溶性多糖。估计其平均分子量（M(r)）为18,000。糖和甲基化分析、部分水解以及13C-核磁共振光谱表明，该多糖是一种支链半乳甘露聚糖，其主链由两个结构单元组成；（1→6）连接的α-D-甘露吡喃糖基和α-D-（1→6）-半乳吡喃糖基单元。在体外吞噬试验和体内碳清除试验中，该多糖表现出显著的免疫刺激活性。
