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Analysis of double-stranded oligonucleotides by electrospray mass spectrometry.

作者信息

Bayer E, Bauer T, Schmeer K, Bleicher K, Maier M, Gaus H J

机构信息

Institute of Organic Chemistry, Universitat Tübingen, Germany.

出版信息

Anal Chem. 1994 Nov 15;66(22):3858-63. doi: 10.1021/ac00094a004.

DOI:10.1021/ac00094a004
PMID:7810895
Abstract

Double-stranded oligonucleotides of different lengths and chemical modification have been analyzed by ion spray mass spectrometry. The non-covalent-bonded duplexes can be detected. Therefore, ion spray mass spectrometry is a useful method for investigation of hybridizations of natural and chemically modified oligonucleotides. Since the exact mass of the double strand can be detected, this method can distinguish between specific and nonspecific interaction.

摘要

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