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来自米曲霉MG49的木聚糖酶的纯化及某些性质

Purification and some properties of a xylanase from Aspergillus sydowii MG49.

作者信息

Ghosh M, Nanda G

机构信息

Department of Microbiology, Bose Institute, Calcutta, India.

出版信息

Appl Environ Microbiol. 1994 Dec;60(12):4620-3. doi: 10.1128/aem.60.12.4620-4623.1994.

DOI:10.1128/aem.60.12.4620-4623.1994
PMID:7811101
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC202033/
Abstract

Aspergillus sydowii MG49 produces a 30-kDa exosplitting xylobiohydrolase during growth on xylan. A specific chemical modification and substrate protection analysis of purified xylanase provided evidence that tryptophan and carboxy and amino groups are present at the catalytic site of this enzyme. Thermal inactivation of the xylanase occurs because of irreversible polymolecular aggregation, which is slower in the presence of glycerol.

摘要

烟曲霉MG49在木聚糖上生长时会产生一种30 kDa的外切木二糖水解酶。对纯化的木聚糖酶进行的特定化学修饰和底物保护分析表明，色氨酸、羧基和氨基存在于该酶的催化位点。木聚糖酶的热失活是由于不可逆的多分子聚集，在甘油存在下这种聚集较慢。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9d8/202033/22f2c0ae54e1/aem00029-0416-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9d8/202033/17c918b9b613/aem00029-0415-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9d8/202033/3ed7c6de0bee/aem00029-0415-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9d8/202033/22f2c0ae54e1/aem00029-0416-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9d8/202033/17c918b9b613/aem00029-0415-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9d8/202033/3ed7c6de0bee/aem00029-0415-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9d8/202033/22f2c0ae54e1/aem00029-0416-a.jpg

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本文引用的文献

1
Notes on sugar determination.糖分测定笔记。
J Biol Chem. 1952 Mar;195(1):19-23.
2
Detection of sugars on paper chromatograms.纸色谱上糖的检测
Nature. 1950 Sep 9;166(4219):444-5. doi: 10.1038/166444b0.
3
Amino acid sequence similarities between low molecular weight endo-1,4-beta-xylanases and family H cellulases revealed by clustering analysis.
FEBS Lett. 1993 Apr 26;321(2-3):135-9. doi: 10.1016/0014-5793(93)80094-b.
嗜盐条件和渗透休克下细胞保护的渗透剂特征
J Fungi (Basel). 2021 May 26;7(6):414. doi: 10.3390/jof7060414.
4
Comparative study of genome-wide plant biomass-degrading CAZymes in white rot, brown rot and soft rot fungi.白腐菌、褐腐菌和软腐菌中全基因组植物生物质降解碳水化合物活性酶的比较研究
Mycology. 2017 Dec 24;9(2):93-105. doi: 10.1080/21501203.2017.1419296. eCollection 2018.
5
Plant-polysaccharide-degrading enzymes from Basidiomycetes.担子菌纲植物多糖降解酶
Microbiol Mol Biol Rev. 2014 Dec;78(4):614-49. doi: 10.1128/MMBR.00035-14.
6
The potential hazards of Aspergillus sp. in foods and feeds, and the role of biological treatment: a review.食品和饲料中曲霉属真菌的潜在危害及生物处理的作用：综述
J Microbiol. 2014 Oct;52(10):807-18. doi: 10.1007/s12275-014-4294-7. Epub 2014 Oct 1.
7
Aspergillus enzymes involved in degradation of plant cell wall polysaccharides.参与植物细胞壁多糖降解的曲霉属酶。
Microbiol Mol Biol Rev. 2001 Dec;65(4):497-522, table of contents. doi: 10.1128/MMBR.65.4.497-522.2001.
4
Characterization of the active site and thermostability regions of endoxylanase from Thermoanaerobacterium saccharolyticum B6A-RI.嗜糖热厌氧杆菌B6A-RI内切木聚糖酶活性位点和热稳定性区域的表征
J Bacteriol. 1993 Sep;175(18):5890-8. doi: 10.1128/jb.175.18.5890-5898.1993.
5
Thermostability of beta-xylosidase from Aspergillus sydowii MG49.来自西氏曲霉MG49的β-木糖苷酶的热稳定性
FEBS Lett. 1993 Sep 20;330(3):275-8. doi: 10.1016/0014-5793(93)80887-z.
6
New families in the classification of glycosyl hydrolases based on amino acid sequence similarities.基于氨基酸序列相似性的糖基水解酶分类中的新家族。
Biochem J. 1993 Aug 1;293 ( Pt 3)(Pt 3):781-8. doi: 10.1042/bj2930781.
7
Purification and characterization of a thermostable xylanase from Bacillus stearothermophilus T-6.嗜热脂肪芽孢杆菌T-6中一种耐热木聚糖酶的纯化与特性分析
Appl Environ Microbiol. 1993 Jun;59(6):1725-30. doi: 10.1128/aem.59.6.1725-1730.1993.
8
Purification and some properties of an alkaline xylanase from alkaliphilic Bacillus sp. strain 41M-1.嗜碱芽孢杆菌41M-1碱性木聚糖酶的纯化及某些性质
Appl Environ Microbiol. 1993 Jul;59(7):2311-6. doi: 10.1128/aem.59.7.2311-2316.1993.
9
Characterization and sequence of a Thermomonospora fusca xylanase.嗜热栖热放线菌木聚糖酶的特性及序列分析
Appl Environ Microbiol. 1994 Mar;60(3):763-70. doi: 10.1128/aem.60.3.763-770.1994.
10
Mechanism of protein stabilization by glycerol: preferential hydration in glycerol-water mixtures.甘油对蛋白质的稳定机制：甘油 - 水混合物中的优先水合作用。
Biochemistry. 1981 Aug 4;20(16):4667-76. doi: 10.1021/bi00519a023.