Sherefa K, Sönnerborg A, Steinbergs J, Sällberg M
Department of Immunology, Karolinska Institutet, Huddinge University Hospital, Sweden.
Biochem Biophys Res Commun. 1994 Dec 30;205(3):1658-64. doi: 10.1006/bbrc.1994.2858.
We developed a typing assay for HIV-1 using subtype specific peptides corresponding to the five major subtypes of HIV-1 (A to E). In eight patients serologically subtyped as A (n = 1), B (n = 3), C (n = 3) and E (n = 1) phyllogenetic analysis of sequenced V3 domain DNA completely correlated to the peptide serotyping. Out of 106 HIV-1 seropositive samples of a diverse geographical origin 88 (83%) could be subtyped by the peptide assay. Five were of subtype A, 33 of subtype B, 48 of subtype C, one of subtype D, and one was of subtype E. Swedish patients were mainly of HIV-1 subtype B and Ethiopian patients were mainly of subtype C, confirming the performance of the assay. Furthermore, subtype specific antibodies may persist up to nine years in HIV-1 infected patients though sera close to AIDS diagnosis may be difficult to type.
我们利用与HIV-1的五种主要亚型（A至E）相对应的亚型特异性肽开发了一种HIV-1分型检测方法。在8名血清学亚型分别为A（n = 1）、B（n = 3）、C（n = 3）和E（n = 1）的患者中，对测序的V3结构域DNA进行系统发育分析，结果与肽血清学分型完全相关。在106份来自不同地理区域的HIV-1血清阳性样本中，88份（83%）可通过肽检测法进行亚型分型。其中5份为A亚型，33份为B亚型，48份为C亚型，1份为D亚型，1份为E亚型。瑞典患者主要为HIV-1 B亚型，埃塞俄比亚患者主要为C亚型，证实了该检测方法的有效性。此外，亚型特异性抗体在HIV-1感染患者体内可能持续长达9年，不过接近艾滋病诊断时的血清可能难以分型。
