多菌型麻风病患者对麻风分枝杆菌65-kD热休克蛋白（hsp）缺乏细胞毒性活性。
Lack of cytotoxic activity against Mycobacterium leprae 65-kD heat shock protein (hsp) in multibacillary leprosy patients.
作者信息
de la Barrera S, Fink S, Finiasz M, Minnucci F, Valdez R, Baliña L M, Sasiain M C
机构信息
Departamento Inmunología, Hospitale Gral San Martín, Buenos Aires, Argentina.
出版信息
Clin Exp Immunol. 1995 Jan;99(1):90-7. doi: 10.1111/j.1365-2249.1995.tb03477.x.
Abstract
Cytotoxic T cells play an important role in host defence mechanisms, as well as in the immunopathology of leprosy. In this study, we evaluated whether Mycobacterium leprae hsp18, hsp65 and Myco. tuberculosis hsp71 could induce cytotoxic T cell activity against autologous macrophages pulsed with these hsp. Paucibacillary (PB) patients and normal controls generated more effector cells than multibacillary (MB) patients with all three hsp tested. There was no cross-reactivity between any of the hsp tested. Mycobacterium leprae hsp65 induced cytotoxic responses only in those MB patients undergoing an erythema nodosum leprosum (ENL) episode. Although hsp65 and hsp18 induced similar proliferation in MB patients, a high proportion of these patients did not generate cytotoxic effector cells in response to hsp65. Hence, those T cells reacting to hsp65 may play an important role in the control of Myco. leprae infection.
摘要
细胞毒性T细胞在宿主防御机制以及麻风病的免疫病理学中发挥着重要作用。在本研究中，我们评估了麻风分枝杆菌hsp18、hsp65以及结核分枝杆菌hsp71是否能够诱导细胞毒性T细胞针对用这些热休克蛋白（hsp）脉冲处理的自体巨噬细胞产生活性。在所有三种被检测的热休克蛋白中，少菌型（PB）患者和正常对照产生的效应细胞比多菌型（MB）患者更多。所检测的任何一种热休克蛋白之间均无交叉反应性。麻风分枝杆菌hsp65仅在那些正经历麻风结节性红斑（ENL）发作的多菌型患者中诱导细胞毒性反应。尽管hsp65和hsp18在多菌型患者中诱导了相似的增殖，但这些患者中有很大比例并未针对hsp65产生细胞毒性效应细胞。因此，那些对hsp65起反应的T细胞可能在控制麻风分枝杆菌感染中发挥重要作用。
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