Norgaard J O
Kidney Int. 1976 Mar;9(3):278-85. doi: 10.1038/ki.1976.30.
A method is described for the isolation of kidney glomeruli using centrifugation in a discontinuous Ficoll gradient. The method, applied to rats, rabbits, pigs and man, yields a glomerular fraction of high purity with a tubular contamination of normally less than 2%. From observations by light microscopy on epoxy resin-embedded fractions, one-third to one-fourth of the glomeruli had suffered only slight damage during isolation. In the electron microscope these best preserved glomeruli showed a close morphologic similarity to those of the intact tissue and preliminary experiments have indicated that they are well-suited for further studies of the in vitro behavior of isolated, viable glomeruli.
本文描述了一种使用不连续Ficoll梯度离心法分离肾肾小球的方法。该方法应用于大鼠、兔子、猪和人类，可获得高纯度的肾小球组分，肾小管污染通常低于2%。通过光学显微镜对环氧树脂包埋组分的观察，三分之一到四分之一的肾小球在分离过程中仅受到轻微损伤。在电子显微镜下，这些保存最好的肾小球与完整组织的肾小球在形态上非常相似，初步实验表明，它们非常适合进一步研究分离的活肾小球的体外行为。
