McCausland I P, Seelye R N, Gavin J B, Herdson P B
Pathology. 1976 Jan;8(1):73-80. doi: 10.3109/00313027609094427.
Scanning electron microscopy showed that most glomeruli isolated by sieving from normal and nephrotoxic rats were cleanly decapsulated and undisrupted. An anti-IgG antibody-horesradish peroxidase conjugate was applied to such isolated glomeruli and also to slices of renal cortex which were sebsequently embedded in epoxy resin. Linear staining along the glomerular basement membranes of nephrotoxic rats was evident and subendothelial electron-dense deposits were shown to contain anti-glomerular basement membrane antibody. Whereas the linear reaction was faint and limited to superficial parts of glomeruli in tissue slices, it was intense and present in most regions of all of the isolated glomeruli. Thus, the fine details of the distribution of intraglomerular antibody are more clearly and consistently demonstrated in isolated glomeruli than in kidney slices.
扫描电子显微镜显示，通过筛分从正常大鼠和肾毒性大鼠分离出的大多数肾小球被干净地剥去包膜且未受破坏。将抗IgG抗体-辣根过氧化物酶偶联物应用于此类分离出的肾小球以及随后包埋在环氧树脂中的肾皮质切片。肾毒性大鼠肾小球基底膜上的线性染色明显，并且显示内皮下电子致密沉积物含有抗肾小球基底膜抗体。虽然在组织切片中线性反应微弱且仅限于肾小球的表层部分，但在所有分离出的肾小球的大多数区域中反应强烈且存在。因此，与肾切片相比，在分离出的肾小球中更清楚且一致地显示出肾小球内抗体分布的精细细节。
