Ho Y M, Ng M H, Huang C T
J Clin Pathol. 1979 Apr;32(4):399-405. doi: 10.1136/jcp.32.4.399.
Two major antigenic components, I and II, were detected by double immunodiffusion in sonic extracts of the germinating (G) or yeast (Y) cells of the dimorphis organism, Candida albicans group A. Component I may be a heterogeneous mixture of antigens which are stable to heating and phenol. Component II is more homogeneous but is labile to heat and phenol. Rabbit antisera, showing only precipitin to component II or certain human sera at high dilution, were found to react with G cells to give an immunofluorescence which was confined to the germ tubes. This suggested that component II is localised on the germ tubes, whereas no immunofluorescent reaction against the yeast cells could be detected under the same conditions although component II was as readily extracted from these cells as from G cells. This suggested that component II might exist in a cryptic state in the Y cells. In support of the latter contention it was shown that live Y cells did not absorb precipitin to component II nor were they capable of providing these antibodies in rabbits. Using both human and rabbit sera, it was shown that the antigenic specificity of the immunofluorescence assay where Y cells were used was related to component I and that where G cells were used it was related to both components I and II.
通过双向免疫扩散法在双态生物体白色念珠菌A组发芽（G）细胞或酵母（Y）细胞的超声提取物中检测到两种主要抗原成分，即成分I和成分II。成分I可能是对加热和苯酚稳定的抗原异质混合物。成分II更均一，但对热和苯酚不稳定。发现仅对成分II产生沉淀素的兔抗血清或高稀释度的某些人血清与G细胞反应，产生局限于芽管的免疫荧光。这表明成分II定位于芽管上，而在相同条件下，尽管成分II从酵母细胞中提取的难易程度与从G细胞中提取的相同，但未检测到针对酵母细胞的免疫荧光反应。这表明成分II可能在Y细胞中以隐匿状态存在。为支持后一种观点，研究表明活的Y细胞既不吸收针对成分II的沉淀素，也不能在兔体内产生这些抗体。使用人血清和兔血清均表明，使用Y细胞时免疫荧光测定的抗原特异性与成分I有关，而使用G细胞时则与成分I和成分II均有关。
