Camilleri J P, Amat C, Chousterman M, Petite J P, Duboust A, Boddaert A, Paraf A
Virchows Arch A Pathol Anat Histol. 1977 Dec 8;376(4):329-41. doi: 10.1007/BF00432302.
A series of 180, Bouin-fixed and paraffin embedded liver biopsies obtained from 147 patients was investigated for the presence of hepatitis B surface antigen (HBs) by histochemical and indirect immunofluorescence techniques. A comparison between orcein staining and Masson's trichrome preparations for ground glass hepatocytes, showed that immunofluorescence was both the more reliable and the more specific method for detection of HBsAg in liver tissue. The ability to perform this technique on paraffin sections facilitates systematic studies and allows retrospective work-up. IF-HBs positive hepatocytes were found in approximately two thirds of all HBs-positive patients in their serum, but never seen in HBs-negative patients. HBs-positive cells were observed in healthy chronic carriers and in all forms of chronic hepatitis, but never in acute HBs-positive hepatitis. In patients treated with chronic hemodialysis and in renal homograft recipients, the incidence of positive cells was higher than in the chronic hepatitis groups; this could be correlated with the duration of antigenemia at the time of biopsy.
采用组织化学和间接免疫荧光技术，对147例患者的180份经Bouin固定、石蜡包埋的肝活检组织进行了乙型肝炎表面抗原（HBs）检测。对用于检测毛玻璃样肝细胞的地衣红染色和Masson三色染色进行比较，结果显示免疫荧光是检测肝组织中HBsAg更可靠、更特异的方法。在石蜡切片上进行该技术的能力有助于系统研究，并允许进行回顾性分析。在血清HBs阳性的所有患者中，约三分之二发现IF-HBs阳性肝细胞，但在HBs阴性患者中从未发现。在健康慢性携带者和所有形式的慢性肝炎中均观察到HBs阳性细胞，但在急性HBs阳性肝炎中从未观察到。在接受慢性血液透析治疗的患者和肾移植受者中，阳性细胞的发生率高于慢性肝炎组；这可能与活检时抗原血症的持续时间有关。
