Camargo Z P, Baruzzi R G, Maeda S M, Floriano M C
Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo (UNIFESP), SP, Brazil.
Med Mycol. 1998 Dec;36(6):413-7.
Lobomycosis and paracoccidioidomycosis are two different mycoses caused by Loboa loboi and Paracoccidioides brasiliensis, respectively. To verify cross-antigenicity between them, lobomycosis sera were tested by immunoblotting, ELISA and capture-EIA against crude exo-antigen, 'cell-free antigen' and gp43 from P. brasiliensis. The majority of lobomycosis serum samples recognized crude exo-antigens and gp43 from P. brasiliensis. Gp43 was eluted from an affinity column prepared with IgG from a patient with active lobomycosis. In lower frequencies and intensities, lobomycosis sera also recognized proteins of 29 kDa, 36 kDa, 39 kDa, 52 kDa, 63 kDa, 70 kDa, 83 kDa, and 108 kDa from P. brasiliensis.
芽生菌病样肉芽肿病和副球孢子菌病是分别由巴西芽生菌和巴西副球孢子菌引起的两种不同的真菌病。为了验证它们之间的交叉抗原性，采用免疫印迹法、酶联免疫吸附测定法（ELISA）和捕获酶免疫测定法，用芽生菌病样肉芽肿病血清检测巴西副球孢子菌的粗制外抗原、“无细胞抗原”和gp43。大多数芽生菌病样肉芽肿病血清样本可识别巴西副球孢子菌的粗制外抗原和gp43。用来自一名活动性芽生菌病样肉芽肿病患者的IgG制备亲和柱，洗脱得到gp43。芽生菌病样肉芽肿病血清还以较低的频率和强度识别巴西副球孢子菌的29 kDa、36 kDa、39 kDa、52 kDa、63 kDa、70 kDa、83 kDa和108 kDa蛋白。
