Read S C, Irwin R J, Poppe C, Harris J
Agriculture and Agri-Food, Canada, Health of Animals Laboratory, Guelph, Ontario.
Poult Sci. 1994 Oct;73(10):1617-21. doi: 10.3382/ps.0731617.
Two methods were compared to determine their ability and efficiency in detecting Salmonella in poultry litter samples. Method 1 consisted of pre-enrichment in buffered peptone water (BPW), selective motility enrichment in Modified Semisolid Rappaport Vassiliadis (MSRV) agar, and plating onto MacConkey (MC) agar. Method 2 employed tetrathionate brilliant green (TBG) broth and plating on brilliant green agar with novobiocin (BGAN) and on xylose lysine tergitol 4 (XLT4) agar. Method 1 resulted in a significantly higher isolation rate, was cheaper, and was less labor intensive.
比较了两种方法，以确定它们检测家禽粪便样本中沙门氏菌的能力和效率。方法1包括在缓冲蛋白胨水（BPW）中进行预富集，在改良半固体Rappaport Vassiliadis（MSRV）琼脂中进行选择性动力富集，并接种到麦康凯（MC）琼脂上。方法2使用四硫磺酸钠煌绿（TBG）肉汤，并接种到含新生霉素的煌绿琼脂（BGAN）和木糖赖氨酸去氧胆酸盐4（XLT4）琼脂上。方法1的分离率显著更高，成本更低，且劳动强度更小。
