Eghianruwa K I, Eyre P
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Canada.
Res Vet Sci. 1994 Sep;57(2):159-62. doi: 10.1016/0034-5288(94)90052-3.
Calf ears which were sensitised to horse serum and others which were unsensitised were catheterised and perfused at a constant flow rate of 2 ml min-1 with Krebs-Henseleit solution using a peristalic pump. The ear preparations were then challenged with whole horse serum, the sensitising antigen. Vascular responses were monitored as changes in perfusion pressure. Weight changes were determined to indicate the extent of oedema formation. Unsensitised ear preparations responded to 0.1 ml whole horse serum with 43.3 +/- 0.8 per cent (n = 5) and 10.7 +/- 0.3 per cent (n = 5) increases in perfusion pressure and weight, respectively. Sensitised ear preparations responded to 0.05 ml whole horse serum by increases of 280.8 +/- 32.9 per cent (n = 5) and 27.2 +/- 1.4 per cent (n = 5) in perfusion pressure and bodyweight, respectively. Isoproterenol (10(-5) M) inhibited the vascular responses by 81.4 +/- 7.6 per cent (n = 5) while the increases in weight were reduced by 20.0 +/- 1.5 per cent (n = 5). The Schultz-Dale reaction has thus been demonstrated in an isolated whole organ, the pinna.
将对马血清敏感的小牛耳朵和未致敏的小牛耳朵插入导管，并用蠕动泵以2毫升/分钟的恒定流速用克雷布斯-亨塞尔特溶液灌注。然后用致敏抗原全马血清对耳部制剂进行激发。监测血管反应作为灌注压力的变化。测定重量变化以表明水肿形成的程度。未致敏的耳部制剂对0.1毫升全马血清的反应分别是灌注压力增加43.3±0.8%（n = 5）和重量增加10.7±0.3%（n = 5）。致敏的耳部制剂对0.05毫升全马血清的反应分别是灌注压力增加280.8±32.9%（n = 5）和体重增加27.2±1.4%（n = 5）。异丙肾上腺素（10^-5 M）抑制血管反应81.4±7.6%（n = 5），而重量增加减少20.0±1.5%（n = 5）。因此，舒尔茨-戴尔反应已在一个孤立的全器官耳廓中得到证实。
