Eghianruwa K I, Eyre P
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Ontario, Canada.
Vet Res Commun. 1991;15(2):117-25. doi: 10.1007/BF00405143.
Bovine ears were catheterized and perfused with Krebs-Henseleit (K-H) solution at a constant flow rate. The perfusion pressure (PP) was monitored using a pressure transducer coupled to a carrier preamplifier, amplifier and a pen recorder. Dose-response curves to phenylephrine, histamine and 5-hydroxytryptamine were obtained. The ear preparations responded to these agonists dose-dependently by increases in perfusion pressure. Ear preparations from sensitized calves were challenged with 0.05 ml of horse serum which caused increased perfusion pressure. These preliminary results suggest that the perfused bovine ear represents a promising, simple, inexpensive model for the study of pharmacological mechanisms of the skin.
将牛耳插管，并以恒定流速用克雷布斯 - 亨塞尔特（K - H）溶液灌注。使用与载波前置放大器、放大器和笔录仪相连的压力传感器监测灌注压力（PP）。获得了去氧肾上腺素、组胺和5 - 羟色胺的剂量反应曲线。耳部制剂对这些激动剂的反应是灌注压力剂量依赖性增加。用0.05 ml马血清刺激致敏小牛的耳部制剂，导致灌注压力升高。这些初步结果表明，灌注牛耳是一种用于研究皮肤药理机制的有前景、简单且廉价的模型。
