Kylmäniemi M, Autio P, Oikarinen A
Department of Oral Surgery, University of Oulu, Finland.
Acta Derm Venereol. 1994 Sep;74(5):364-7. doi: 10.2340/0001555574364367.
Levels of interleukin 1 alpha (IL-1 alpha) were studied from blister fluids collected from 14 patients with various types of blistering diseases. In all the fluids, IL-1 alpha could be detected, the concentrations varying from 5 to 1730 pg/ml. For comparison IL-1 alpha was also assayed from suction blisters of 13 subjects; 8 atopic patients and 5 healthy controls. IL-1 alpha was also present in suction blisters in measurable quantities, suggesting that during suction IL-1 alpha is released into the blister cavity. Since IL-1 alpha has been shown to have marked effects on collagen metabolism, the marker of collagen synthesis (carboxyterminal propeptide of type I procollagen (PICP)) and gelatinase were assayed from the same samples. There was no apparent correlation between the levels of IL-1 alpha, PICP or gelatinase in blister fluids. The possible association of IL-1 alpha and collagen metabolism was further studied in experimental conditions. Topical glucocorticoid markedly decreased the level of PICP in suction blisters but did not have any significant effect on IL-1 alpha. UVB-radiation, on the other hand, caused increase in IL-1 alpha but did not have any profound effect on collagen metabolism. During the re-epithelialization of the blister floor the level of IL-1 alpha decreased markedly, and at the same time the expression of gelatinase was increased. The results indicate that IL-1 alpha is released in large quantities into blister fluid when using the suction blister model. However, no apparent correlation could be observed in healthy or diseased skin between the levels of IL-1 alpha, collagen synthesis marker or gelatinase.
对14例患有各种水疱性疾病患者的疱液进行白细胞介素1α（IL-1α）水平研究。在所有疱液中均能检测到IL-1α，其浓度在5至1730 pg/ml之间变化。为作比较，还对13名受试者的抽吸疱进行了IL-1α检测；其中8名特应性患者和5名健康对照者。抽吸疱中也存在可测量量的IL-1α，这表明在抽吸过程中IL-1α释放到疱腔内。由于IL-1α已被证明对胶原蛋白代谢有显著影响，因此从相同样本中检测了胶原蛋白合成标志物（I型前胶原羧基末端前肽（PICP））和明胶酶。疱液中IL-1α、PICP或明胶酶水平之间无明显相关性。在实验条件下进一步研究了IL-1α与胶原蛋白代谢的可能关联。局部使用糖皮质激素显著降低了抽吸疱中PICP的水平，但对IL-1α无任何显著影响。另一方面，紫外线辐射导致IL-1α增加，但对胶原蛋白代谢无任何深远影响。在疱底重新上皮化过程中，IL-1α水平显著下降，同时明胶酶的表达增加。结果表明，使用抽吸疱模型时，IL-1α大量释放到疱液中。然而，在健康或患病皮肤中，未观察到IL-1α水平、胶原蛋白合成标志物或明胶酶之间有明显相关性。
