Schmidt E, Mittnacht A, Schömig H, Dummer R, Bröcker E B, Zillikens D
Department of Dermatology, Univeristy of Würzburg, Germany.
J Dermatol Sci. 1996 Feb;11(2):142-7. doi: 10.1016/0923-1811(95)00435-1.
Bullous pemphigoid (BP) is an autoimmune subepidermal blistering disease. In addition to autoantibodies, complement activation and inflammatory cells are necessary for lesion formation. In this study, we investigated the potential involvement of IL-1, secreted by various inflammatory and non-inflammatory cells, in BP lesions. We determined IL-1 alpha IL-1 beta and IL-1 receptor antagonist in both blister fluid and concurrent serum samples of 10 BP patients by ELISAs. For comparison, we assayed experimentally generated suction blisters from 10 healthy volunteers. IL-1 beta levels were significantly elevated in BP blisters, whereas levels of IL-1 alpha were decreased relative to those in controls. In concurrent serum samples, no IL-1 alpha or IL-1 beta were detected in patients or controls. Levels of IL-1 receptor antagonist were significantly higher in BP blisters in relation to both concurrent sera and suction blisters. Our data indicate the release of IL-1 beta, IL-1 receptor antagonist, and to a lesser extent, of IL-1 alpha, at the site of blister formation in BP and support the notion that cell-mediated immune mechanisms may contribute to blister formation in this disease.
大疱性类天疱疮（BP）是一种自身免疫性表皮下疱病。除自身抗体外，补体激活和炎性细胞对于皮损形成也是必需的。在本研究中，我们调查了由各种炎性和非炎性细胞分泌的白细胞介素-1（IL-1）在BP皮损中的潜在作用。我们通过酶联免疫吸附测定法（ELISA）测定了10例BP患者水疱液及同期血清样本中的IL-1α、IL-1β和IL-1受体拮抗剂。作为对照，我们检测了10名健康志愿者实验性产生的抽吸疱。BP水疱中IL-1β水平显著升高，而IL-1α水平相对于对照组降低。在同期血清样本中，患者和对照组均未检测到IL-1α或IL-1β。与同期血清和抽吸疱相比，BP水疱中IL-1受体拮抗剂水平显著更高。我们的数据表明，在BP水疱形成部位有IL-1β、IL-1受体拮抗剂释放，以及程度较轻的IL-1α释放，并支持细胞介导的免疫机制可能参与该疾病水疱形成的观点。
