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聚合酶链反应用于监测医务人员在使用二氧化碳激光和电凝治疗尖锐湿疣过程中的人乳头瘤病毒污染情况。

Polymerase chain reaction for monitoring human papillomavirus contamination of medical personnel during treatment of genital warts with CO2 laser and electrocoagulation.

作者信息

Bergbrant I M, Samuelsson L, Olofsson S, Jonassen F, Ricksten A

机构信息

Department of Dermatology and Venereology, University of Göteborg, Sweden.

出版信息

Acta Derm Venereol. 1994 Sep;74(5):393-5. doi: 10.2340/0001555574393395.

DOI:10.2340/0001555574393395
PMID:7817682
Abstract

Genital warts and intraepithelial neoplasia caused by infection with human papillomavirus are usually treated with CO2 laser or electrocoagulation. In this study, contamination of personnel and operating theatres with human papillomavirus DNA during treatment sessions was investigated. Samples were taken from the nostrils, nasolabial folds and conjunctiva of the operating physician before and after operating sessions and from Petri dishes left open in the operating theatres. Human papillomavirus DNA was demonstrated by the polymerase chain reaction technique. The results show that there is a risk of contamination of the operator by human papillomavirus DNA, detectable with the polymerase chain reaction technique, during both CO2 laser and electrocoagulation treatment.

摘要

由人乳头瘤病毒感染引起的尖锐湿疣和上皮内瘤变通常采用二氧化碳激光或电凝治疗。在本研究中,对治疗期间人乳头瘤病毒DNA污染工作人员和手术室的情况进行了调查。在手术前后采集手术医生的鼻孔、鼻唇沟和结膜样本,以及手术室中敞口放置的培养皿样本。采用聚合酶链反应技术检测人乳头瘤病毒DNA。结果表明,在二氧化碳激光和电凝治疗过程中,存在操作人员被人乳头瘤病毒DNA污染的风险,采用聚合酶链反应技术可检测到这种污染。

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