Yang J H, Lee H C, Lin K J, Wei Y H
Department of Dermatology, China Medical College and Hospital, Taichung, Taiwan.
Arch Dermatol Res. 1994;286(7):386-90. doi: 10.1007/BF00371798.
Mitochondrial DNA (mtDNA) mutations have recently been proposed as important contributors to ageing and degenerative diseases. In this study, we applied the polymerase chain reaction technique to detect 4.977 bp-deleted mtDNA, the most common and abundant deletion of mtDNA, in normal human skin. None of the skin specimens from patients less than 60 years of age were found to bear this type of mtDNA deletion. The incidence of the 4977-bp-deleted mtDNA increased with advancing age. The incidence of the deletion was 20%, 46.7% and 83.4% for patients of the age groups of 61-70, 71-80, and 81-90 years, respectively. Moreover, the incidence of 4977-bp-deleted mtDNA was significantly higher in exposed skin than that in non-exposed skin. We suggest that mtDNA mutations are molecular events associated with the human skin ageing process.
线粒体DNA（mtDNA）突变最近被认为是衰老和退行性疾病的重要成因。在本研究中，我们应用聚合酶链反应技术检测正常人皮肤中4977bp缺失的mtDNA，这是mtDNA最常见且数量最多的缺失类型。在60岁以下患者的皮肤样本中，未发现携带这种类型的mtDNA缺失。4977bp缺失的mtDNA的发生率随年龄增长而增加。61 - 70岁、71 - 80岁和81 - 90岁年龄组患者的缺失发生率分别为20%、46.7%和83.4%。此外，暴露皮肤中4977bp缺失的mtDNA的发生率显著高于非暴露皮肤。我们认为mtDNA突变是与人类皮肤衰老过程相关的分子事件。
