Curiale M S, Sons T, Fanning L, Lepper W, McIver D, Garramone S, Mozola M
Silliker Laboratories Group, Inc., Chicago Heights, IL 60411.
J AOAC Int. 1994 May-Jun;77(3):602-17.
The method is based on the hybridization of synthetic deoxyribonucleic acid probes to ribosomal ribonucleic acid sequences unique to Listeria. This method was compared to 2 culture methods: the U.S. Food and Drug Administration method for the detection of Listeria in dairy products and seafoods and the U.S. Department of Agriculture, Food Safety and Inspection Service method for Listeria in meats. Six food types with replicate samples containing various concentrations of Listeria were analyzed by the collaborating laboratories. Listeria was detected in 774 samples using the DNAH method and in 772 samples using a culture method. The DNAH and culture methods were in agreement for 668 samples containing Listeria and 306 samples without Listeria. The overall rate of agreement between methods was 82.3%. The method has been adopted first action by AOAC INTERNATIONAL.
该方法基于合成脱氧核糖核酸探针与李斯特菌特有的核糖体核糖核酸序列的杂交。此方法与两种培养方法进行了比较：美国食品药品监督管理局用于检测乳制品和海产品中李斯特菌的方法，以及美国农业部食品安全与检验局用于检测肉类中李斯特菌的方法。合作实验室对六种含有不同浓度李斯特菌的重复样本的食品类型进行了分析。使用DNAH方法在774个样本中检测到了李斯特菌，使用培养方法在772个样本中检测到了李斯特菌。DNAH方法和培养方法在668个含有李斯特菌的样本和306个不含李斯特菌的样本上结果一致。两种方法的总体一致率为82.3%。该方法已被美国官方分析化学师协会首次采用。
