Musk S R, Astley S B, Edwards S M, Stephenson P, Hubert R B, Johnson I T
Institute of Food Research, Norwich Laboratory, Colney, UK.
Food Chem Toxicol. 1995 Jan;33(1):31-7. doi: 10.1016/0278-6915(95)80245-2.
Benzyl isothiocyanate (BITC), a compound found in cruciferous vegetables present in the human diet, has previously been shown to induce chromosome aberrations in an Indian muntjac cell line. The results of this study show that it also induces both chromosome aberrations and sister chromatid exchanges (SCEs) in Chinese hamster ovary (CHO) cells in the absence of an exogenous metabolic activation system and induces DNA strand breaks as measured by the single-cell gel electrophoresis assay. However, whereas it increased the number of aberrations four-fold, it was not able to raise SCE levels by more than 50% and there was a levelling-off in the dose-response curve. Whereas the survival curve of CHO cells exposed to BITC was linear in shape, that of the human colorectal adenocarcinoma cell line HT29 was found to fit the exponential model (with an alpha equivalent of 0.28 and a beta equivalent of 2.80, where the concentration of BITC is measured in micrograms/ml). This pattern of clastogenic and cytotoxic activities is reminiscent of that generated by ionizing radiation and certain radiomimetic chemotherapeutic agents.
苄基异硫氰酸酯（BITC）是一种存在于人类饮食中的十字花科蔬菜中的化合物，此前已被证明能在印度麂细胞系中诱导染色体畸变。本研究结果表明，在没有外源性代谢激活系统的情况下，它也能在中国仓鼠卵巢（CHO）细胞中诱导染色体畸变和姐妹染色单体交换（SCE），并通过单细胞凝胶电泳测定法诱导DNA链断裂。然而，虽然它使畸变数量增加了四倍，但它不能使SCE水平提高超过50%，并且剂量反应曲线出现了平稳。暴露于BITC的CHO细胞的存活曲线呈线性，而人结肠直肠腺癌细胞系HT29的存活曲线则符合指数模型（α相当于0.28，β相当于2.80，其中BITC的浓度以微克/毫升为单位）。这种致断裂和细胞毒性活性模式让人联想到电离辐射和某些拟放射化疗药物所产生的模式。
