Musk S R, Smith T K, Johnson I T
Institute of Food Research, Norwich Laboratory, Colney, UK.
Mutat Res. 1995 Sep;348(1):19-23. doi: 10.1016/0165-7992(95)90016-0.
Four compounds commonly found in the human diet, allyl isothiocyanate (AITC), phenethyl isothiocyanate (PEITC) and their parent glucosinolates sinigrin and gluconasturtiin, were tested for cytotoxic and genotoxic effects in a Chinese hamster ovary cell line (CHO). The isothiocyanates were found to be more than one thousand times more cytotoxic than the glucosinolates, showing significant cytotoxic activity at concentrations below 1.0 microgram/ml. AITC was unable to induce either chromosome aberrations or sister chromatid exchanges (SCEs) even at highly cytotoxic doses. In contrast, PEITC was found to induce both aberrations and SCE at concentrations of 0.9-1.2 micrograms/ml whilst sinigrin and gluconasturtiin induced aberrations at concentrations above 2 mg/ml.
对人类饮食中常见的四种化合物，即烯丙基异硫氰酸酯（AITC）、苯乙基异硫氰酸酯（PEITC）及其母体硫代葡萄糖苷黑芥子硫苷酸钾和葡糖芥苷，在中国仓鼠卵巢细胞系（CHO）中进行了细胞毒性和遗传毒性作用测试。发现异硫氰酸酯的细胞毒性比硫代葡萄糖苷大一千多倍，在浓度低于1.0微克/毫升时显示出显著的细胞毒性活性。即使在高细胞毒性剂量下，AITC也无法诱导染色体畸变或姐妹染色单体交换（SCE）。相比之下，发现PEITC在浓度为0.9 - 1.2微克/毫升时可诱导畸变和SCE，而黑芥子硫苷酸钾和葡糖芥苷在浓度高于2毫克/毫升时可诱导畸变。
