Masuoka K, Sagawa K, Mochizuki M, Oizumi K, Itoh K
Department of Immunology, Kurume University School of Medicine, Japan.
Invest Ophthalmol Vis Sci. 1995 Jan;36(1):254-8.
To understand better the immunopathology of HTLV-I uveitis by investigating the clonality of HTLV-I-infected T-cell clones.
Eleven T-cell clones were established from the aqueous humor (six clones) and the peripheral blood (five clones) of a patient with HTLV-I uveitis, and the clonality of the HTLV-I-infected T cells was investigated by sequencing the T-cell receptor (TCR) alpha gene after the amplification of TCR alpha cDNA using an adaptor-ligation method and reverse transcriptase-polymerase chain reaction (RT-PCR).
TCR alpha use was different for each of 11 T-cell clones, encompassing eight different HTLV-I-infected T-cell clones (four from the aqueous humor and four from peripheral blood) and three HTLV-I-negative T-cell clones.
This study demonstrated polyclonal use of TCR alpha for HTLV-I-infected T cells in the ocular lesion and the peripheral blood. Results suggested that these T cells are not precursors of the leukemic cells associated with malignant transformation. Instead, they might be randomly infected with HTLV-I in the process of HTLV-I uveitis.
通过研究人类嗜T淋巴细胞病毒I型（HTLV-I）感染的T细胞克隆的克隆性，更好地理解HTLV-I葡萄膜炎的免疫病理学。
从一名HTLV-I葡萄膜炎患者的房水（6个克隆）和外周血（5个克隆）中建立了11个T细胞克隆，并在使用衔接子连接法和逆转录聚合酶链反应（RT-PCR）扩增T细胞受体（TCR）α cDNA后，通过对TCRα基因进行测序来研究HTLV-I感染的T细胞的克隆性。
11个T细胞克隆中的每一个克隆对TCRα的使用都不同，包括8个不同的HTLV-I感染的T细胞克隆（4个来自房水，4个来自外周血）和3个HTLV-I阴性T细胞克隆。
本研究证明了在眼部病变和外周血中HTLV-I感染的T细胞对TCRα的多克隆使用。结果表明，这些T细胞不是与恶性转化相关的白血病细胞的前体。相反，它们可能在HTLV-I葡萄膜炎过程中被HTLV-I随机感染。
