Prince A, Neu H C
Antimicrob Agents Chemother. 1976 May;9(5):776-9. doi: 10.1128/AAC.9.5.776.
The basis of ampicillin resistance in Shigella sonnei was analyzed for a number of isolates. Most isolates that were resistant to ampicillin and cephalothin failed to transfer resistance. Isolates resistant to cephalothin and ampicillin were inhibited by mecillinam, cefamandole, and cefoxitin. Six strains were selected for study. These differed on the basis of inhibition by 10 beta-lactam antibiotics and on differences in beta-lactamase activity. There was no correlation between the inhibitory concentration of antibiotic and the amount of antibiotic hydrolyzed. Use of sodium chloride and cloxacillin inhibition and acrylamide electrophoresis demonstrated that ampicillin resistance in Shigella is mediated by a number of different beta-lactamases. Organisms can contain more than one type of beta-lactamase.
对若干宋内志贺菌分离株的氨苄西林耐药性基础进行了分析。大多数对氨苄西林和头孢噻吩耐药的分离株未能传递耐药性。对头孢噻吩和氨苄西林耐药的分离株被美西林、头孢孟多和头孢西丁抑制。选择了6株菌株进行研究。这些菌株在对10种β-内酰胺抗生素的抑制作用以及β-内酰胺酶活性方面存在差异。抗生素的抑制浓度与水解抗生素的量之间没有相关性。使用氯化钠和氯唑西林抑制以及丙烯酰胺电泳表明，志贺菌中的氨苄西林耐药性由多种不同的β-内酰胺酶介导。生物体可含有不止一种类型的β-内酰胺酶。
