Yao Z, Seelig H P, Ehrfeld H, Renz M, Hartung K, Deicher H, Keller E, Nevinny-Stickel C, Albert E D
Labor für Immungenetik, Universität München, Germany.
Rheumatol Int. 1994;14(2):63-9. doi: 10.1007/BF00300249.
To investigate a possible involvement of HLA-class II alleles in the genetic predisposition for the formation of anti-U1-nRNP antibody-in systemic lupus erythematosus (SLE), genomic DNA of 178 patients was typed for the DRB1, DQA1 and DQB1 alleles using a polymerase chain reaction (PCR) and non-radioactive-oligonucleotide typing. Antibodies against recombinant U1-nRNP proteins (U1-A-, U1-C- and 70K-protein) were determined by ELISA. Anti-U1-C antibody was found in 26 (14.7%), anti-U1-A in 34 (19.2%) and anti-70K in 17 (9.6%) patients. A joint occurrence was observed for these antibodies against the recombinant U1-nRNP proteins: anti-U1-C and anti-U1-A antibodies occurred together more frequently than alone and than together with anti-U1-70K antibodies. The frequency of DRB104 was slightly increased in the patients with anti-U1-C as compared to the patients without anti-U1-C (P < 0.05, Pcorr = n.s., RR = 2.4). The DQA10301 allele, which is in linkage disequilibrium with DRB104, is found more frequently in anti-U1-C-positive than in antibody-negative patients. The DQB10303 allele, detected in 12 of 176 SLE patients, was absent in the patients with any of the antibodies against the U1-nRNP proteins. All these deviations may be due to chance alone. We concluded that the presence of antibodies against recombinant U1-nRNP proteins was not significantly associated with any HLA DRB1, DQA1 and DQB1 allele in our group of SLE patients.
为研究人类白细胞抗原（HLA）-Ⅱ类等位基因是否可能参与系统性红斑狼疮（SLE）中抗U1核糖核蛋白（nRNP）抗体形成的遗传易感性，采用聚合酶链反应（PCR）和非放射性寡核苷酸分型法，对178例患者的基因组DNA进行DRB1、DQA1和DQB1等位基因分型。采用酶联免疫吸附测定法（ELISA）检测针对重组U1-nRNP蛋白（U1-A、U1-C和70K蛋白）的抗体。在26例（14.7%）患者中发现抗U1-C抗体，34例（19.2%）患者中发现抗U1-A抗体，17例（9.6%）患者中发现抗70K抗体。观察到这些针对重组U1-nRNP蛋白的抗体同时出现：抗U1-C和抗U1-A抗体同时出现的频率高于单独出现的频率，也高于与抗U1-70K抗体同时出现的频率。与无抗U1-C抗体的患者相比，抗U1-C抗体阳性患者中DRB104的频率略有增加（P<0.05，校正P值无统计学意义，相对危险度RR=2.4）。与DRB104处于连锁不平衡状态的DQA10301等位基因，在抗U1-C抗体阳性患者中的出现频率高于抗体阴性患者。在176例SLE患者中有12例检测到DQB10303等位基因，在任何一种抗U1-nRNP蛋白抗体阳性的患者中均未检测到该等位基因。所有这些差异可能仅为偶然因素所致。我们得出结论，在我们的SLE患者组中，针对重组U1-nRNP蛋白的抗体的存在与任何HLA DRB1、DQA1和DQB1等位基因均无显著相关性。
