西奈芬净通过共享腺苷甲硫氨酸摄取系统进入杜氏利什曼原虫前鞭毛体。
Sinefungin shares AdoMet-uptake system to enter Leishmania donovani promastigotes.
作者信息
Phelouzat M A, Basselin M, Lawrence F, Robert-Gero M
机构信息
Institut de Chimie des Substances Naturelles, C.N.R.S., Gif-sur Yvette, France.
出版信息
Biochem J. 1995 Jan 1;305 ( Pt 1)(Pt 1):133-7. doi: 10.1042/bj3050133.
Abstract
The involvement of a carrier for sinefungin (SF) uptake in Leishmania donovani promastigotes is indicated by saturation kinetics, competition studies and SF accumulation against a 270-fold concentration gradient across the cell membrane. Whether SF uptake occurs via nucleoside- or AdoMet-carrier systems was investigated by competition experiments and comparison of the uptake of various molecules in wild-type and SF-resistant cells. Results show that SF did not inhibit purine or pyrimidine uptake whereas it competitively inhibited AdoMet uptake. Furthermore, the uptake of nucleosides in SF-resistant cells is similar to that in wild-type cells, whereas uptake of SF and AdoMet is lower.
摘要
饱和动力学、竞争研究以及抗利什曼原虫前鞭毛体中抗杀稻瘟菌素（SF）摄取的载体参与情况表明，SF能逆着细胞膜上270倍的浓度梯度进行积累。通过竞争实验以及对野生型和抗SF细胞中各种分子摄取情况的比较，研究了SF的摄取是通过核苷载体系统还是通过腺苷甲硫氨酸（AdoMet）载体系统进行的。结果表明，SF并不抑制嘌呤或嘧啶的摄取，而竞争性抑制AdoMet的摄取。此外，抗SF细胞中核苷的摄取与野生型细胞中的相似，而SF和AdoMet的摄取较低。
相似文献
1
Sinefungin shares AdoMet-uptake system to enter Leishmania donovani promastigotes.西奈芬净通过共享腺苷甲硫氨酸摄取系统进入杜氏利什曼原虫前鞭毛体。
Biochem J. 1995 Jan 1;305 ( Pt 1)(Pt 1):133-7. doi: 10.1042/bj3050133.
2
Leishmania donovani: antagonistic effect of S-adenosyl methionine on ultrastructural changes and growth inhibition induced by sinefungin.杜氏利什曼原虫：S-腺苷甲硫氨酸对杀稻瘟菌素诱导的超微结构变化和生长抑制的拮抗作用
Exp Parasitol. 1992 Mar;74(2):177-87. doi: 10.1016/0014-4894(92)90045-c.
3
Distribution of macromolecular methylations in promastigotes of Leishmania donovani and impact of sinefungin.杜氏利什曼原虫前鞭毛体中大分子甲基化的分布及杀稻瘟菌素的影响
J Eukaryot Microbiol. 1993 Sep-Oct;40(5):581-9. doi: 10.1111/j.1550-7408.1993.tb06111.x.
4
Characterization of sinefungin-resistant Leishmania donovani promastigotes.抗杀稻瘟菌素的杜氏利什曼原虫前鞭毛体的特性研究
Parasitol Res. 1993;79(8):683-9. doi: 10.1007/BF00932511.
5
Proton-ATPase activities involved in the uptake of an S-adenosylmethionine analogue.参与S-腺苷甲硫氨酸类似物摄取的质子-ATP酶活性。
Mol Biochem Parasitol. 1998 Apr 1;92(1):99-109. doi: 10.1016/s0166-6851(97)00235-1.
6
Expression of the major surface glycoprotein of Leishmania, gp63, in wild-type and sinefungin-resistant promastigotes.利什曼原虫主要表面糖蛋白gp63在野生型和抗杀稻瘟菌素前鞭毛体中的表达。
Eur J Biochem. 1994 Jul 1;223(1):61-8. doi: 10.1111/j.1432-1033.1994.tb18966.x.
7
Pentamidine uptake in Leishmania donovani and Leishmania amazonensis promastigotes and axenic amastigotes.喷他脒在杜氏利什曼原虫和亚马逊利什曼原虫前鞭毛体及无菌无鞭毛体中的摄取情况。
Biochem J. 1996 Apr 15;315 ( Pt 2)(Pt 2):631-4. doi: 10.1042/bj3150631.
8
Point mutations in a nucleoside transporter gene from Leishmania donovani confer drug resistance and alter substrate selectivity.来自杜氏利什曼原虫的核苷转运蛋白基因中的点突变赋予耐药性并改变底物选择性。
Proc Natl Acad Sci U S A. 2001 May 22;98(11):6092-7. doi: 10.1073/pnas.101537298. Epub 2001 May 15.
9
Two high affinity nucleoside transporters in Leishmania donovani.杜氏利什曼原虫中的两种高亲和力核苷转运体。
Mol Biochem Parasitol. 1987 Jan 2;22(1):29-37. doi: 10.1016/0166-6851(87)90066-1.
10
Characterization of a methionine adenosyltransferase over-expressing strain in the trypanosomatid Leishmania donovani.杜氏利什曼原虫中甲硫氨酸腺苷转移酶过表达菌株的特性分析
Biochim Biophys Acta. 2006 Jan;1760(1):10-9. doi: 10.1016/j.bbagen.2005.09.003. Epub 2005 Sep 22.
引用本文的文献
1
Polyamine Metabolism in Parasites: A Promising Therapeutic Target.寄生虫中的多胺代谢：有前途的治疗靶点。
Med Sci (Basel). 2022 Apr 22;10(2):24. doi: 10.3390/medsci10020024.
2
Genomewide Analysis of Mode of Action of the -Adenosylmethionine Analogue Sinefungin in Leishmania infantum.婴儿利什曼原虫中S-腺苷甲硫氨酸类似物杀稻瘟菌素作用模式的全基因组分析。
mSystems. 2019 Oct 15;4(5):e00416-19. doi: 10.1128/mSystems.00416-19.
3
Genetic mapping reveals that sinefungin resistance in Toxoplasma gondii is controlled by a putative amino acid transporter locus that can be used as a negative selectable marker.遗传图谱分析表明，弓形虫对杀稻瘟菌素的抗性由一个假定的氨基酸转运基因座控制，该基因座可作为负选择标记。
Eukaryot Cell. 2015 Feb;14(2):140-8. doi: 10.1128/EC.00229-14. Epub 2014 Dec 5.
4
High affinity S-Adenosylmethionine plasma membrane transporter of Leishmania is a member of the folate biopterin transporter (FBT) family.利什曼原虫高亲和力 S-腺苷甲硫氨酸质膜转运蛋白是叶酸生物蝶呤转运体 (FBT) 家族的成员。
J Biol Chem. 2010 Jun 25;285(26):19767-75. doi: 10.1074/jbc.M110.114520. Epub 2010 Apr 20.
5
Sinefungin resistance of Saccharomyces cerevisiae arising from Sam3 mutations that inactivate the AdoMet transporter or from increased expression of AdoMet synthase plus mRNA cap guanine-N7 methyltransferase.酿酒酵母对杀稻瘟菌素的抗性源于使AdoMet转运蛋白失活的Sam3突变，或源于AdoMet合酶加mRNA帽鸟嘌呤-N7甲基转移酶表达增加。
Nucleic Acids Res. 2007;35(20):6895-903. doi: 10.1093/nar/gkm817. Epub 2007 Oct 11.
6
TOR-induced resistance to toxic adenosine analogs in Leishmania brought about by the internalization and degradation of the adenosine permease.TOR诱导利什曼原虫对有毒腺苷类似物产生抗性，这是由腺苷通透酶的内化和降解所导致的。
Exp Cell Res. 2007 May 15;313(9):1963-78. doi: 10.1016/j.yexcr.2007.02.027. Epub 2007 Mar 12.
7
Nucleoside and nucleobase transporters in parasitic protozoa.寄生原生动物中的核苷和核碱基转运蛋白。
Eukaryot Cell. 2004 Apr;3(2):245-54. doi: 10.1128/EC.3.2.245-254.2004.
8
Antitrypanosomal activity of purine nucleosides can be enhanced by their conversion to O-acetylated derivatives.嘌呤核苷转化为O-乙酰化衍生物后，其抗锥虫活性可得到增强。
Antimicrob Agents Chemother. 1996 Nov;40(11):2567-72. doi: 10.1128/AAC.40.11.2567.
本文引用的文献
1
Uptake and metabolism of S-adenosyl-L-methionine by Leishmania mexicana and Leishmania braziliensis promastigotes.墨西哥利什曼原虫和巴西利什曼原虫前鞭毛体对S-腺苷-L-甲硫氨酸的摄取与代谢
Mol Biochem Parasitol. 1993 Mar;58(1):123-34. doi: 10.1016/0166-6851(93)90096-g.
2
Distribution of macromolecular methylations in promastigotes of Leishmania donovani and impact of sinefungin.杜氏利什曼原虫前鞭毛体中大分子甲基化的分布及杀稻瘟菌素的影响
J Eukaryot Microbiol. 1993 Sep-Oct;40(5):581-9. doi: 10.1111/j.1550-7408.1993.tb06111.x.
3
Characterization of sinefungin-resistant Leishmania donovani promastigotes.抗杀稻瘟菌素的杜氏利什曼原虫前鞭毛体的特性研究
Parasitol Res. 1993;79(8):683-9. doi: 10.1007/BF00932511.
4
Inhibition of sterol transmethylation by S-adenosylhomocysteine analogs.S-腺苷同型半胱氨酸类似物对甾醇转甲基作用的抑制
J Bacteriol. 1981 Jan;145(1):106-12. doi: 10.1128/jb.145.1.106-112.1981.
5
Inhibitory activity of sinefungin and SIBA (5'-deoxy-5'-S-isobutylthio-adenosine) on the growth of promastigotes and amastigotes of different species of Leishmania.西奈芬净和SIBA（5'-脱氧-5'-S-异丁硫基腺苷）对不同利什曼原虫物种前鞭毛体和无鞭毛体生长的抑制活性。
FEBS Lett. 1980 Dec 1;121(2):287-91. doi: 10.1016/0014-5793(80)80364-4.
6
An in-vitro system for determining the activity of compounds against the intracellular amastigote form of Leishmania donovani.一种用于测定化合物对杜氏利什曼原虫细胞内无鞭毛体形式活性的体外系统。
J Antimicrob Chemother. 1984 Nov;14(5):463-75. doi: 10.1093/jac/14.5.463.
7
A9145, a new adenine-containing antifungal antibiotic. I. Discovery and isolation.A9145，一种新型含腺嘌呤抗真菌抗生素。I. 发现与分离
J Antibiot (Tokyo). 1973 Aug;26(8):463-5. doi: 10.7164/antibiotics.26.463.
8
Differential effect of sinefungin and its analogs on the multiplication of three Leishmania species.杀稻瘟菌素及其类似物对三种利什曼原虫增殖的差异效应。
Antimicrob Agents Chemother. 1985 Oct;28(4):528-31. doi: 10.1128/AAC.28.4.528.
9
Anti-leishmanial effect of allopurinol ribonucleoside and the related compounds, allopurinol, thiopurinol, thiopurinol ribonucleoside, and of formycin B, sinefungin and the lepidine WR6026.别嘌呤醇核糖核苷及相关化合物别嘌呤醇、硫嘌呤醇、硫嘌呤醇核糖核苷、以及间型霉素B、杀稻瘟菌素和勒皮定WR6026的抗利什曼原虫作用。
Trans R Soc Trop Med Hyg. 1985;79(1):122-8. doi: 10.1016/0035-9203(85)90255-x.
10
Two high affinity nucleoside transporters in Leishmania donovani.杜氏利什曼原虫中的两种高亲和力核苷转运体。
Mol Biochem Parasitol. 1987 Jan 2;22(1):29-37. doi: 10.1016/0166-6851(87)90066-1.
Zotero 插件