Phelouzat M A, Basselin M, Lawrence F, Robert-Gero M
Institut de Chimie des Substances Naturelles, C.N.R.S., Gif-sur Yvette, France.
Biochem J. 1995 Jan 1;305 ( Pt 1)(Pt 1):133-7. doi: 10.1042/bj3050133.
The involvement of a carrier for sinefungin (SF) uptake in Leishmania donovani promastigotes is indicated by saturation kinetics, competition studies and SF accumulation against a 270-fold concentration gradient across the cell membrane. Whether SF uptake occurs via nucleoside- or AdoMet-carrier systems was investigated by competition experiments and comparison of the uptake of various molecules in wild-type and SF-resistant cells. Results show that SF did not inhibit purine or pyrimidine uptake whereas it competitively inhibited AdoMet uptake. Furthermore, the uptake of nucleosides in SF-resistant cells is similar to that in wild-type cells, whereas uptake of SF and AdoMet is lower.
饱和动力学、竞争研究以及抗利什曼原虫前鞭毛体中抗杀稻瘟菌素（SF）摄取的载体参与情况表明，SF能逆着细胞膜上270倍的浓度梯度进行积累。通过竞争实验以及对野生型和抗SF细胞中各种分子摄取情况的比较，研究了SF的摄取是通过核苷载体系统还是通过腺苷甲硫氨酸（AdoMet）载体系统进行的。结果表明，SF并不抑制嘌呤或嘧啶的摄取，而竞争性抑制AdoMet的摄取。此外，抗SF细胞中核苷的摄取与野生型细胞中的相似，而SF和AdoMet的摄取较低。
